Colorimetric Determination of Sulfide in Microsamples

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  • SUGAHARA Shogo
    Graduate School of Science and Engineering, Shimane University
  • SUZUKI Mai
    Graduate School of Science and Engineering, Shimane University
  • KAMIYA Hiroshi
    Shimane Prefectural Institute of Public Health and Environmental Science
  • YAMAMURO Masumi
    Institute of Geology and Geoinformation, Geological Survey of Japan (GSJ), AIST
  • SEMURA Hitoshi
    Inland Water Fisheries and Coastal Fisheries Division, Shimane Prefectural Fisheries Technology Center
  • SENGA Yukiko
    Faculty of Science, Toho University
  • EGAWA Michiko
    Graduate School of Science and Engineering, Shimane University
  • SEIKE Yasushi
    Graduate School of Science and Engineering, Shimane University

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Abstract

A method for determination of hydrogen sulfide in microsamples (200 μL) was developed by modifying the methylene blue method. Samples were collected using a micropipette and were combined with sulfide coloring reagent and 5 mL of 0.1 M HCl in test tubes. Absorbance of the solution was measured spectrophotometrically at 667 nm. This modified method did not require any special labware or technique, and can be used in a variety of research fields.

Journal

  • Analytical Sciences

    Analytical Sciences 32 (10), 1129-1131, 2016

    The Japan Society for Analytical Chemistry

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