Microglial phospholipase D4 deficiency influences myelination during brain development

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Author(s)

    • CHIBA Terumasa
    • Department of Molecular Neurobiology, Tokyo University of Pharmacy and Life Sciences
    • OTANI Yoshinori
    • Department of Molecular Neurobiology, Tokyo University of Pharmacy and Life Sciences
    • YAMAGUCHI Yoshihide
    • Department of Molecular Neurobiology, Tokyo University of Pharmacy and Life Sciences
    • ISHIBASHI Tomoko
    • Department of Molecular Neurobiology, Tokyo University of Pharmacy and Life Sciences
    • HAYASHI Akiko
    • Department of Molecular Neurobiology, Tokyo University of Pharmacy and Life Sciences
    • YAMAZAKI Maya
    • Department of Cellular Neurobiology, Brain Research Institute, Niigata University
    • SAKIMURA Kenji
    • Department of Cellular Neurobiology, Brain Research Institute, Niigata University
    • BABA Hiroko
    • Department of Molecular Neurobiology, Tokyo University of Pharmacy and Life Sciences

Abstract

<p>Phospholipase D4 (PLD4) is expressed in activated microglia that transiently appear in white matter during postnatal brain development. Previous knockdown experiments using cultured microglia showed PLD4 involvement in phagocytosis and proliferation. To elucidate the role of PLD4 <i>in vivo</i>, PLD4-deficient mice were generated and the cerebella were examined at postnatal day 5 (P5) and P7, when PLD4 expression is highest in microglia. Wild type microglia showed strong immunoreactivity for microglial marker CD68 at P5, whereas CD68 signals were weak in PLD4-deficient microglia, suggesting that loss of PLD4 affects microglial activation. At P5 and P7, immunostaining for anti-myelin basic protein (MBP) antibody indicated a mild but significant delay in myelination in PLD4-deficient cerebellum. Similar change was also observed in the corpus callosum at P7. However, this difference was not apparent at P10, suggesting that microglial PLD4-deficiency primarily influences the early myelination stage. Thus, microglia may have a transient role in myelination via a PLD4-related mechanism during development.</p>

Journal

  • Proceedings of the Japan Academy, Series B

    Proceedings of the Japan Academy, Series B 92(7), 237-254, 2016

    The Japan Academy

Codes

  • NII Article ID (NAID)
    130005253188
  • Text Lang
    ENG
  • ISSN
    0386-2208
  • Data Source
    J-STAGE 
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