Effects of Selenium Deficiency on Proteins Containing Essential Trace Elements (Fe, Cu, Zn, Mn, Se) in Mouse Brain

• ABIKO Takuto

  Faculty of Science and Engineering, Department of Applied Chemistry, Chuo University

• KOBAYASHI Keito

  Faculty of Science and Engineering, Department of Applied Chemistry, Chuo University

• MATSUKAWA Takehisa

  Faculty of Medicine, Department of Epidemiology and Environmental Health, Juntendo University

• SHINOHARA Atsuko

  Faculty of Medicine, Department of Epidemiology and Environmental Health, Juntendo University

• FURUTA Naoki

  Faculty of Science and Engineering, Department of Applied Chemistry, Chuo University

Bibliographic Information

Other Title

• セレン欠乏によるマウス脳内の必須微量元素（Fe, Cu, Zn, Mn, Se）を含むタンパク質への影響
• セレン ケツボウ ニ ヨル マウス ノウナイ ノ ヒッス ビリョウ ゲンソ(Fe,Cu,Zn,Mn,Se)オ フクム タンパクシツ エ ノ エイキョウ

Abstract

After selenium-82 isotope-enriched selenite (⁸²Se(IV)) or selenomethionine (⁸²SeMet) was intravenously injected to mice fed with selenium adequate diets (Se adequate mice) and to mice fed with selenium deficient diets (Se deficient mice), speciation analysis of essential trace elements (Fe, Cu, Zn, Mn, Se) containing proteins in brain was conducted by using inductive coupled plasma mass spectrometry (ICPMS) combined with high-performance liquid chromatography (HPLC). In Se deficient mice, endogenous selenium was kept constant due to homeostasis, and selenium stored as a chemical form of selenoprotein P (Sel-P) was used to produce cellular glutathione peroxidase (cGPx). Injected Se was not used to produce Sel-P, but used to produce cGPx. cGPx was more efficiently produced by SeMet injection than Se(IV) injection. When Se was deficient in the brain, complementary reactions to reduce superoxide were observed. Superoxide dismutase (SOD) and metallothionein (MT), which play an important role in antioxidant, were produced more compared with Se adequate mice, whereas cytochrome c (Cyt c), which produces hydroxyl radical, was less produced compared with Se adequate mice. Particularly large increase of endogenous cGPx, SOD and MT was observed after Se(IV) injection compared with SeMet injection. That implies antioxidant proteins were required because superoxide were more produced when Se(IV) was injected. From these experimental results, it could be considered that essential elements (Fe, Cu, Zn, Mn, Se) containing proteins complementarily worked to reduce damage of brain cells from active oxygen.

Journal

• BUNSEKI KAGAKU

  BUNSEKI KAGAKU 65 (7), 371-378, 2016

  The Japan Society for Analytical Chemistry

Keywords

• HPLC-ICPMS
• selenium deficient
• glutathione peroxidase
• superoxide dismutase
• metallothionein
• cytochrome <i>c</i>

Details

• CRID: 1390282679638212096
• NII Article ID: 130005253341
• NII Book ID: AN00222633
• DOI: 10.2116/bunsekikagaku.65.371
• NDL BIB ID: 027566096
• ISSN: 05251931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027566096
• Web Site: https://www.jstage.jst.go.jp/article/bunsekikagaku/65/7/65_371/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016322223
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed