Splitting-free Bioluminescence Imaging Probes and Their Applications
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- KIM Sung Bae
- Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST)
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- FUJII Rika
- Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST)
Bibliographic Information
- Other Title
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- 非分割型生物発光イメージングプローブの開発と応用
- 分析化学総説 非分割型生物発光イメージングプローブの開発と応用
- ブンセキ カガク ソウセツ ヒブンカツガタ セイブツ ハッコウ イメージングプローブ ノ カイハツ ト オウヨウ
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Abstract
Molecular imaging is recited as a key technology to access the molecular events inside living subjects and is thus a primary research interest among the fields of medicine, pharmacy, biology, environmental science, and chemistry. Molecular imaging provides an ideal toolbox to tackle such multidisciplinary issues including investigation of pathological phenomena, development of new drugs, evaluation of toxicity, etc. However, noninvasive access to such molecular events is a mission impossible as the molecular events occur temporally and do not accompany any detectable optical radiation. During the past decades, a dramatic progress has been achieved in the imaging technology with fluorescent proteins and luciferases, called "reporter". This technology may be categorized into two major groups: (1) a method to temporarily split the reporter protein into two fragments and reconstitute them conditionally, called protein-fragment complementation assay (PCA), and (2) a method to make use of the full length of the reporters as is, i.e., splitting-free. The authors highlight the later molecular imaging strategy utilizing full-length reporter proteins.
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 65 (7), 361-369, 2016
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679638213376
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- NII Article ID
- 130005253342
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- NII Book ID
- AN00222633
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- NDL BIB ID
- 027566074
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- ISSN
- 05251931
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed