頭頸部癌における酸化ストレスの評価  [in Japanese] Evaluation of Oxidative Stress in Head and Neck Carcinoma  [in Japanese]

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Author(s)

    • 高橋 克昌 Takahashi Katsumasa
    • 群馬大学大学院医学系研究科耳鼻咽喉科・頭頸部外科学 Department of Otorhinolaryngology-Head and Neck Surgery, Gunma University Graduate School of Medicine
    • 高安 幸弘 Takayasu Yukihiro
    • 群馬大学大学院医学系研究科耳鼻咽喉科・頭頸部外科学 Department of Otorhinolaryngology-Head and Neck Surgery, Gunma University Graduate School of Medicine
    • 近松 一朗 Chikamatsu Kazuaki
    • 群馬大学大学院医学系研究科耳鼻咽喉科・頭頸部外科学 Department of Otorhinolaryngology-Head and Neck Surgery, Gunma University Graduate School of Medicine

Abstract

<b>背景・目的:</b>生体において酸化と抗酸化のバランスが崩れ, 活性酸素が蓄積する状態を酸化ストレスと言う. 癌や感染症では, 白血球の産生する過剰な活性酸素のために酸化ストレスが蓄積する. 癌の治療前後で酸化ストレスの状態を評価し, 白血球との関連を検討した.<br><b>対象と方法:</b>10名の頭頸部癌患者の抗癌剤投与前・後と回復期において, 血清中の酸化ストレス (dROM, diacron-Reactive Oxygen Metabolite) と抗酸化力 (BAP, biological antioxidant potential) を測定した. 10名の中耳炎患者の手術前・後と回復期で同様の測定をし, 両者を比較検討した. dROMは活性酸素により酸化された血清のヒドロペルオキシド濃度で, 酸化ストレスの指標とした. BAPは血清が還元した3価の鉄イオンを含む呈色液の濃度で, 抗酸化力の指標とした.<br><b>結 果:</b>癌は中耳炎と比較して有意にdROMが高値を示した. BAPは両者に差を認めなかった. 治療によって癌ではdROMが変化したが, 白血球数の減少と一致しなかった. 中耳炎ではdROMは一定で, 白血球数の増加に伴った変化も認めなかった.<br><b>結 語:</b>中耳炎の手術前後でdROMが上昇しなかったことから, 局所炎症や全身麻酔手術程度では酸化ストレスと抗酸化力のバランスが崩れないと考えた. 逆に治療前から高いdROMを示した癌は, 常に強い酸化ストレス状態にあると推測された. 治療により白血球が減少してもdROMの低下は伴わず, 白血球以外で活性酸素を放出すると考えられた.

<b>Background and Aim: </b>Oxidative stress is an imbalance between the production and the elimination of oxidant species. In cancer and infection, white blood cells overproduce reactive oxygen species, causing the oxidative stress. In the present study, we evaluated the oxidative stress in patients with cancer, and compared the relationship between the oxidative stress and the number of white blood cells (WBC).<br><b>Subjects and Methods: </b>The subjects were head and neck carcinoma patients (HNC) who were treated by chemotherapy (n=10), and blood tests were achieved before and after treatment. Otitis media patients (OM) who had surgery were also applied (n=10). The diacron-reactive oxygen metabolite (dROM), which indicated the concentration of hydroperoxides in serum, was measured as an index of oxidative stress. The biological antioxidant potential (BAP), which indicated the concentration of deoxidized iron ion in serum, was also measured as an index of anti-oxidative status.<br><b>Results: </b>The dROM was significantly high in HNC, although dROM in OM was normal. There was no difference in BAP between HNC and OM. In HNC, WBC decreased significantly after chemotherapy, but changes in dROM did not show a particular trend. There was no relationship between them. In OM, WBC tended to increase after surgery, but dROM did not change.<br><b>Conclusions: </b>The dROM did not increase after OM surgery, meaning that general anesthesia and surgery did not change the balance of oxidative stress and antioxidant potential. In HNC, a strong oxidative stress was present, as dROM was very high even before treatment. The dROM stayed high, whereas WBC decreased after chemotherapy. Reactive oxygen species in HNC patients might not be produced by WBC.

Journal

  • The Kitakanto Medical Journal

    The Kitakanto Medical Journal 66(2), 117-121, 2016

    The Kitakanto Medical Society

Codes

  • NII Article ID (NAID)
    130005253710
  • NII NACSIS-CAT ID (NCID)
    AN10585677
  • Text Lang
    JPN
  • Article Type
    journal article
  • ISSN
    1343-2826
  • NDL Article ID
    027458222
  • NDL Call No.
    Z19-138
  • Data Source
    NDL  IR  J-STAGE 
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