Effect of Seed Sludge on Survival of Antibiotic-Resistant Bacteria and Fate of Bacteria Resistant to Different Classes of Antibiotics during Anaerobic Digestion of Dairy Manure

• Takemura Toshiki

  Department of Agricultural Engineering and Socio-Economics, Kobe University, Kobe, Japan

• Ihara Ikko

  Department of Agricultural Engineering and Socio-Economics, Kobe University, Kobe, Japan

• Kitazono Yumika

  Department of Agricultural Engineering and Socio-Economics, Kobe University, Kobe, Japan

• Toyoda Kiyohiko

  Department of Agricultural Engineering and Socio-Economics, Kobe University, Kobe, Japan

• Iwasaki Masahiro

  Department of Animal Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

• Umetsu Kazutaka

  Department of Animal Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

Abstract

Livestock manure is one of main pollution reservoirs of antibiotic-resistant bacteria (ARBs) due to excessive use of veterinary antibiotics. For preventing the environmental spread, an efficient process is required to reduce ARBs in livestock manure. In this work, we investigated the survival of ARB during mesophilic anaerobic digestion of dairy cattle manure. Effects of seed sludge and the antibiotic class were investigated for reduction of ARBs by batch digestion tests. The numbers of all ARBs were 6.34×10⁴ to 2.08×10⁶ before anaerobic digestion. The numbers of oxytetracycline, chlortetracycline, gentamicin and kanamycin -resistant bacteria decreased close to the detection limit after 20-day anaerobic digestion. However, reduction effects of cefazolin and streptomycin -resistant bacteria were small. The seed sludge had little influence on the survival of ARBs during the anaerobic digestion. The results showed that the ARB reduction effect was influenced by the types of antibiotics, and mostly not by seed sludge.

Journal

• Journal of Water and Environment Technology

  Journal of Water and Environment Technology 14 (4), 282-288, 2016

  Japan Society on Water Environment

Keywords

• antibiotic-resistant bacteria
• anaerobic digestion
• seed sludge.

Details

• CRID: 1390001205178483072
• NII Article ID: 130005259662
• DOI: 10.2965/jwet.15-070
• ISSN: 13482165
• Web Site: https://www.jstage.jst.go.jp/article/jwet/14/4/14_15-070/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed