抽出物がDNAであることを確かめる対照実験を伴う検証の必要性  [in Japanese] The Need for Inspection of the Control Experiment to Verify that an Extract is DNA in High School Biology Classes  [in Japanese]

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Abstract

<p>近年, 生物の授業において, DNA抽出実験が, 頻繁に実施されるようになった. しかし, 現在実施されている実験形式では, 抽出物がDNAである(DNAを含んでいる)ことを納得できない生徒がいると指摘されており, 試薬を用いて抽出物がDNAであることを確かめる過程を導入することが必要と言える. そこで, 本研究では, 従来のDNA抽出実験の操作に, 対照実験を伴う検証過程を導入し, その導入が抽出物はDNAであると納得することに繋がるかどうかを明らかにすることを目的とした. 質問紙調査の結果, 検証前では抽出物がDNAであると納得できなかった生徒が全体の約1/3を占めたが, 検証後では, それらの生徒も, 抽出物がDNAであると納得したことから, DNA抽出実験における試薬を用いた検出の重要性が明らかになった. その際, 特に対照実験を伴う方がより生徒の納得につながることが明らかになった. </p>

<p>In recent years, DNA extraction experiments have come to be carried out frequently in biology classes at upper secondary schools. However, it is said that in the way the experiments are currently carried out, some students cannot verify that an extract is DNA (or include DNA). Therefore, it is necessary to introduce a process to check that an extract is DNA by using a reagent. In this study, we introduced an inspection process with the control experiment into the operation process of the conventional DNA extraction experiment and investigated whether the introduction of the inspection process was connected to improved understanding that the extract was DNA. As a result of the survey, about 1/3 of the students were not able to understand that an extract was DNA before the inspection. However, after the inspection, all the students became convinced that an extract was DNA. From this result, the importance of the detection using the reagent in the DNA extraction experiment has become clear. This research has shows that the use of the inspection process with the control experiment will help students verify and understand. </p>

Journal

  • Journal of Research in Science Education

    Journal of Research in Science Education 57(1), 63-70, 2016

    Society of Japan Science Teaching

Codes

  • NII Article ID (NAID)
    130005260498
  • NII NACSIS-CAT ID (NCID)
    AA11406090
  • Text Lang
    JPN
  • ISSN
    1345-2614
  • NDL Article ID
    027568805
  • NDL Call No.
    Z7-1282
  • Data Source
    NDL  J-STAGE 
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