Mn（II）イオンの結合によって誘起される結晶相ADPリボース加水分解酵素反応の観察

• 古池 美彦

  自然科学研究機構分子科学研究所協奏分子システム研究センター

• 宮原 郁子

  大阪市立大学大学院理学研究科

• 神谷 信夫

  大阪市立大学複合先端研究機構

書誌事項

タイトル別名

• Observation of ADP-Ribose Pyrophosphatase Reaction in Crystalline-State Induced by Mn（II）Ion Binding
• Mn(Ⅱ)イオン ノ ケツゴウ ニ ヨッテ ユウキ サレル ケッショウソウ ADP リボース カスイブンカイ コウソ ハンノウ ノ カンサツ

抄録

ADP-Ribose pyrophosphatase (ADPRase) reaction was observed in crystalline-state at atomic resolutions around 1 Å. ADPRase achieves the general acid-base catalysis in the presence of divalent metal cations. Crystals of ADPRase alone, ADPRase-ADPR binary (ES-state) complex were prepared, and reaction intermediates were captured by the cryo-trapping after the soaking of MnCl₂ into ES-state crystals. Just after two metal binding sites were successively occupied along with the soaking time, ADPR was hydrolyzed and reaction products were released, indicating the completion of the crystalline-state reaction. The key structures of ADPRase-ADPR-two Mn²⁺ ions complex revealed the various enzymatic phenomena including electrostatic, proximity and orientation effects and preferential binding for the transition states. The details of the metal assisting mechanism and the general base catalysis have been discussed.

収録刊行物

• 日本結晶学会誌

  日本結晶学会誌 58 (4), 186-192, 2016

  日本結晶学会

詳細情報

• CRID: 1390001204088166272
• NII論文ID: 130005261612
• NII書誌ID: AN00188364
• DOI: 10.5940/jcrsj.58.186
• ISSN: 18845576
• ISSN: 03694585
• NDL書誌ID: 027620273
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027620273
• Web Site: https://www.jstage.jst.go.jp/article/jcrsj/58/4/58_186/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可