培養神経回路網の形成過程における自発性神経活動と培養時のブドウ糖濃度の関係性

• 箕嶋 渉

  関西学院大学理工学研究科人間システム工学専攻神経知能工学研究室（工藤研究室） 関西学院大学バイオ・ロボティクス研究センター

• 伊東 嗣功

  関西学院大学理工学研究科人間システム工学専攻神経知能工学研究室（工藤研究室） 関西学院大学バイオ・ロボティクス研究センター

• 工藤 卓

  関西学院大学理工学研究科人間システム工学専攻神経知能工学研究室（工藤研究室） 関西学院大学バイオ・ロボティクス研究センター

書誌事項

タイトル別名

• Relationship between Autonomous Activity in Cultured Neuronal Networks and Glucose Concentration of Culture Condition
• バイヨウ シンケイ カイロモウ ノ ケイセイ カテイ ニ オケル ジハツセイ シンケイ カツドウ ト バイヨウジ ノ ブドウトウ ノウド ノ カンケイセイ

抄録

Cultured rat hippocampal network on a multi electrodes array (MEA) dish is useful for analyzing the relationships between electrical activity and culture condition during developing the networks. Seeded neurons autonomously form a complex network on a MEA dish and autonomous electrical activity is often observed without any inputs from outer world. Autonomous activity is considered to reflect internal states of the neuronal network. We elucidated that there are a particular relationship between autonomous activity and glucose concentration of culture medium at 7.5 mM, 17.5 mM and 30 mM during developing neuronal network. In the case of 17.5 mM glucose concentration, We confirmed that the frequency of autonomous electrical spikes increased accompany with culture days. In the case of 30 mM glucose concentration, trend of autonomous activity was similar to the case of 17.5 mM. Thus, in the case of 30 mM glucose concentration, glucose neurotoxicity was not obviously observed. Also in the case of 7.5 mM glucose concentration, frequency of autonomous electrical spikes similarly increased during culture days, however, the frequency was significantly low comparing width the case of 17.5 mM glucose concentration. In addition, the frequency of electrical spikes in the condition of 7.5 mM glucose concentration and the initial density of 7800 cells / mm² was similar to the frequency of the spikes in neuronal networks cultured at the initial density of 2600 cells / mm² or 3900 cells / mm² in 17.5 mM glucose concentration. These results show that neurons adjust the culture size and electrical activity to the glucose concentration of culture medium during developing a neuronal network.

収録刊行物

• 電気学会論文誌Ｃ（電子・情報・システム部門誌）

  電気学会論文誌Ｃ（電子・情報・システム部門誌） 136 (9), 1335-1342, 2016

  一般社団法人 電気学会

キーワード

• 培養神経回路網
• 多電極アレイ
• 自発性神経活動
• 細胞外ブドウ糖濃度

詳細情報

• CRID: 1390001204607157504
• NII論文ID: 130005262358
• NII書誌ID: AN10065950
• DOI: 10.1541/ieejeiss.136.1335
• ISSN: 13488155
• ISSN: 03854221
• NDL書誌ID: 027650781
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027650781
• Web Site: https://www.jstage.jst.go.jp/article/ieejeiss/136/9/136_1335/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可