Significance of regulating protein functions under hypothermic oxygenated conditions

DOI
  • Fukai Moto
    Department of Transplant Surgery, Graduate School of Medicine, Hokkaido University
  • Shimada Shingo
    Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University
  • Kobayashi Nozomi
    Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University
  • Ishikawa Takahisa
    Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University
  • Umemoto Kohei
    Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University
  • Ohtani Shintaro
    Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University
  • Yamashita Kenichiro
    Department of Transplant Surgery, Graduate School of Medicine, Hokkaido University
  • Shimamura Tsuyoshi
    Division of Organ Transplantation, Hokkaido University Hospital
  • Taketomi Akinobu
    Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University

Bibliographic Information

Other Title
  • 低温酸素化状態におけるタンパク機能制御

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Abstract

Cellular survival and death during cold storage and hypothermic perfusion are regulated by intracellular signal, however, precise mechanisms remain elusive. Although the effector proteins of cellular fate work actually during 6 to 24 hours after rewarming and reoxygenation, the triggering events have already been regulated during organ procurement, transportation, and implantation before reperfusion. In this review, we summarize some potential mechanisms of regulating intracellular signals, mainly focused on the energy production and post-translational regulation under hypothermic conditions.<br>A chaperon protein, 14-3-3ζ, regulates over 200 of the protein activity by covering the phosphorylation site within a 14-3-3 binding motif. Sirtuins also regulates many proteins directly and indirectly relates to the cell survival and death under some difficult conditions including cold and/or hypoxic insults. Sirtuins regulate many mitochondrial proteins primarily by deacetylation instead of ATP consuming processes. Further, sirtuins and 14-3-3s interact each other and sharing some target proteins, especially relating energy production and mitochondrial functions. Although these facts have been reported in ectotherms, hibernators, to human, little is known about its role in organ preservation, perfusion, and transplantation. Here, we reviewed its potential significance for the future research in the field of organ repair.

Journal

  • Organ Biology

    Organ Biology 23 (2), 173-179, 2016

    The Japan Society for Organ Preservation and Biology

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