Vital Staining of Acidic Compartments in Brown Alga <i>Desmarestia Viridis</i> Cells

DOI
  • SASAKI Hideaki
    College of Science and Engineering, Iwaki Meisei University
  • IWATA Eri
    College of Science and Engineering, Iwaki Meisei University

抄録

Desmarestia viridis(Phaeophyceae)accumulate a highly acidic substance in the vegetative cells of the thallus. Prior attempts to clarify the intracellular locality of the acidic substance have been inconclusive until now. As a result of D. viridis cell observation using an optical microscope, neutral red indicating an acidic compartment accumulated in vacuoles. Vital staining by the pH indicator bromophenol blue showed the strong acid accumulated in the vacuole. Furthermore, methyl violet showed intracellural pH was approximately 1.0, and accorded with past results that were derived from inorganic ion concentration.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390001204596480384
  • NII論文ID
    130005264604
  • DOI
    10.11457/swsj.69.118
  • ISSN
    21859213
    03694550
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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