H<sub>2</sub>-Producing Bacterial Community during Rice Straw Decomposition in Paddy Field Soil: Estimation by an Analysis of [FeFe]-Hydrogenase Gene Transcripts

Access this Article

Author(s)

    • Baba Ryuko
    • Laboratory of Soil Biology and Chemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
    • Asakawa Susumu
    • Laboratory of Soil Biology and Chemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
    • Watanabe Takeshi
    • Laboratory of Soil Biology and Chemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

Abstract

<p>The transcription patterns of [FeFe]-hydrogenase genes (<i>hydA</i>), which encode the enzymes responsible for H<sub>2</sub> production, were investigated during rice straw decomposition in paddy soil using molecular biological techniques. Paddy soil amended with and without rice straw was incubated under anoxic conditions. RNA was extracted from the soil, and three clone libraries of <i>hydA</i> were constructed using RNAs obtained from samples in the initial phase of rice straw decomposition (day 1 with rice straw), methanogenic phase of rice straw decomposition (day 14 with rice straw), and under a non-amended condition (day 14 without rice straw). <i>hydA</i> genes related to <i>Proteobacteria</i>, <i>Firmicutes</i>, <i>Bacteroidetes</i>, <i>Chloroflexi</i>, and <i>Thermotogae</i> were mainly transcribed in paddy soil samples; however, their proportions markedly differed among the libraries. <i>Deltaproteobacteria-</i>related <i>hydA</i> genes were predominantly transcribed on day 1 with rice straw, while various types of <i>hydA</i> genes related to several phyla were transcribed on day 14 with rice straw. Although the diversity of transcribed <i>hydA</i> was significantly higher in the library on day 14 with rice straw than the other two libraries, the composition of <i>hydA</i> transcripts in the library was similar to that in the library on day 14 without rice straw. These results indicate that the composition of active H<sub>2</sub> producers and/or H<sub>2</sub> metabolic patterns dynamically change during rice straw decomposition in paddy soil.</p>

Journal

  • Microbes and Environments

    Microbes and Environments 31(3), 226-233, 2016

    Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology

Codes

  • NII Article ID (NAID)
    130005265124
  • Text Lang
    ENG
  • ISSN
    1342-6311
  • Data Source
    J-STAGE 
Page Top