H₂-Producing Bacterial Community during Rice Straw Decomposition in Paddy Field Soil: Estimation by an Analysis of [FeFe]-Hydrogenase Gene Transcripts

• Baba Ryuko

  Laboratory of Soil Biology and Chemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

• Asakawa Susumu

  Laboratory of Soil Biology and Chemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

• Watanabe Takeshi

  Laboratory of Soil Biology and Chemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

Bibliographic Information

Other Title

• H₂-Producing Bacterial Community during Rice Straw Decomposition in Paddy Field Soil : Estimation by an Analysis of [FeFe]-Hydrogenase Gene Transcripts

Abstract

The transcription patterns of [FeFe]-hydrogenase genes (hydA), which encode the enzymes responsible for H₂ production, were investigated during rice straw decomposition in paddy soil using molecular biological techniques. Paddy soil amended with and without rice straw was incubated under anoxic conditions. RNA was extracted from the soil, and three clone libraries of hydA were constructed using RNAs obtained from samples in the initial phase of rice straw decomposition (day 1 with rice straw), methanogenic phase of rice straw decomposition (day 14 with rice straw), and under a non-amended condition (day 14 without rice straw). hydA genes related to Proteobacteria, Firmicutes, Bacteroidetes, Chloroflexi, and Thermotogae were mainly transcribed in paddy soil samples; however, their proportions markedly differed among the libraries. Deltaproteobacteria-related hydA genes were predominantly transcribed on day 1 with rice straw, while various types of hydA genes related to several phyla were transcribed on day 14 with rice straw. Although the diversity of transcribed hydA was significantly higher in the library on day 14 with rice straw than the other two libraries, the composition of hydA transcripts in the library was similar to that in the library on day 14 without rice straw. These results indicate that the composition of active H₂ producers and/or H₂ metabolic patterns dynamically change during rice straw decomposition in paddy soil.

Journal

• Microbes and Environments

  Microbes and Environments 31 (3), 226-233, 2016

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• paddy field soil
• [FeFe]-hydrogenase
• hydrogen-producing microorganism
• anoxic soil incubation
• clone library analysis

Details

• CRID: 1390282679321930880
• NII Article ID: 40020943031
• NII Article ID: 130005265124
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me16036
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 027619024
• PubMed: 27319579
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027619024
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/31/3/31_ME16036/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed