Identification of Lung Inflammation-Related Elevation of Acylamino Acid Releasing Enzyme (APEH) Activity Using an Enzymomics Approach

• Komatsu Toru

  Graduate School of Pharmaceutical Sciences, The University of Tokyo Precursory Research for Embryonic Science and Technology (PRESTO) Investigator, Japan Science and Technology Agency (JST)

• Yoshioka Kentaro

  Graduate School of Pharmaceutical Sciences, The University of Tokyo

• Hanaoka Kenjiro

  Graduate School of Pharmaceutical Sciences, The University of Tokyo

• Terai Takuya

  Graduate School of Pharmaceutical Sciences, The University of Tokyo

• Ueno Tasuku

  Graduate School of Pharmaceutical Sciences, The University of Tokyo

• Nagano Tetsuo

  Drug Discovery Initiative, The University of Tokyo

• Urano Yasuteru

  Graduate School of Pharmaceutical Sciences, The University of Tokyo Graduate School of Medicine, The University of Tokyo Core Research for Evolutional Science and Technology (CREST) Investigator, Japan Agency for Medical Research and Development (AMED)

Bibliographic Information

Other Title

• Communication to the Editor : Identification of Lung Inflammation-Related Elevation of Acylamino Acid Releasing Enzyme (APEH) Activity Using an Enzymomics Approach

Abstract

Disease-associated alterations of enzymatic functions are potentially useful as disease biomarkers, and here we show that an enzymomics (omics of active enzymes) approach, in which enzymatic activities are screened with panels of substrates, can be an effective way to identify such alterations. In the present study, we used a panel of fluorogenic substrates to search for altered enzyme activities in bronchoalveolar lavage fluid (BALF) from a mouse model of lung inflammation. We found that acylamino acid releasing enzyme (APEH) activity was highly elevated, apparently reflecting the increased population of immune cells in the inflamed lung.

Journal

• Chemical and Pharmaceutical Bulletin

  Chemical and Pharmaceutical Bulletin 64 (11), 1533-1538, 2016

  The Pharmaceutical Society of Japan

Keywords

• chemical biology
• fluorescent probe
• enzymomics
• biomarker
• lung inflammation

Details

• CRID: 1390282679155282688
• NII Article ID: 130005268073
• NII Book ID: AA00602100
• DOI: 10.1248/cpb.c16-00540
• ISSN: 13475223
• ISSN: 00092363
• NDL BIB ID: 027684399
• PubMed: 27803464
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027684399
• Web Site: https://www.jstage.jst.go.jp/article/cpb/64/11/64_c16-00540/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed