Fluidic Culture and Analysis of Pulmonary Artery Smooth Muscle Cells for the Study of Pulmonary Hypertension

• SATO Kae

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University

• NAKAJIMA Minori

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University

• TOKUDA Sana

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University

• OGAWA Aiko

  Division of Molecular and Cellular Medicine, Department of Clinical Science, National Hospital Organization Okayama Medical Center

Abstract

There is an urgent need to develop novel in-vitro models to mimic the disease conditions in pulmonary hypertension (PH). We developed a microfluidic cell culture device for PH studies that withstood high shear stress. Techniques were also developed for cell recovery from the microchannel and mRNA isolation from the collected cells. Using this device, we found that shear stress caused a 7.5-fold increase in the transcription levels of a PH-related molecule, Cyclin D1.

Journal

• Analytical Sciences

  Analytical Sciences 32 (11), 1217-1221, 2016

  The Japan Society for Analytical Chemistry

Keywords

• Microfluidics
• cell culture
• vascular
• shear stress

Details

• CRID: 1390282679238173568
• NII Article ID: 130005277935
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.32.1217
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 027714060
• PubMed: 27829629
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027714060
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.32.1217.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.32.1217/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/32/11/32_1217/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2017176362
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed