A Nanodiamond-peptide Bioconjugate for Fluorescence and ODMR Microscopy of a Single Actin Filament

• GENJO Takuya

  Department of Molecular Engineering, Graduate School of Engineering, Kyoto University

• SOTOMA Shingo

  Department of Molecular Engineering, Graduate School of Engineering, Kyoto University Institute of Atomic and Molecular Sciences, Academia Sinica

• TANABE Ryotaro

  Department of Molecular Engineering, Graduate School of Engineering, Kyoto University

• IGARASHI Ryuji

  Department of Molecular Engineering, Graduate School of Engineering, Kyoto University PRESTO, Japan Science and Technology Agency

• SHIRAKAWA Masahiro

  Department of Molecular Engineering, Graduate School of Engineering, Kyoto University Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University CREST

抄録

Recently, the importance of conformational changes in actin filaments induced by mechanical stimulation of a cell has been increasingly recognized, especially in terms of mechanobiology. Despite its fundamental importance, however, long-term observation of a single actin filament by fluorescent microscopy has been difficult because of the low photostability of traditional fluorescent molecules. This paper reports a novel molecular labeling system for actin filaments using fluorescent nanodiamond (ND) particles harboring nitrogen-vacancy centers; ND has flexible chemical modifiability, extremely high photostability and biocompatibility, and provides a variety of physical information quantitatively via optically detected magnetic resonance (ODMR) measurements. We performed the chemical surface modification of an ND with the actin filament-specific binding peptide Lifeact and observed colocalization of pure Lifeact-modified ND and actin filaments by the ODMR selective imaging protocol, suggesting the capability of long-term observation and quantitative analysis of a single molecule by using an ND particle.

収録刊行物

• Analytical Sciences

  Analytical Sciences 32 (11), 1165-1170, 2016

  社団法人 日本分析化学会

キーワード

• Actin filament
• nanodiamond
• bioimaging
• single molecular observation

詳細情報

• CRID: 1390001204261583232
• NII論文ID: 130005278003
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.32.1165
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 027713948
• PubMed: 27829620
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027713948
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.32.1165.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.32.1165/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/32/11/32_1165/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2017176357
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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