Morphological and Mechanical Properties of Osteosarcoma Microenvironment Cells Explored by Atomic Force Microscopy Morphological and Mechanical Properties of Osteosarcoma Microenvironment Cells Explored by Atomic Force Microscopy

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Author(s)

    • WANG Xinlong WANG Xinlong
    • International Center for Materials Nanoarchitectonics, National Institute for Materials Science|Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba
    • YANG Yingjun YANG Yingjun
    • International Center for Materials Nanoarchitectonics, National Institute for Materials Science|Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba
    • HU Xiaohong HU Xiaohong
    • International Center for Materials Nanoarchitectonics, National Institute for Materials Science|Graduate School of Life and Environmental Science, University of Tsukuba
    • CHEN Guoping CHEN Guoping
    • International Center for Materials Nanoarchitectonics, National Institute for Materials Science|Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba

Abstract

Cell mechanical properties that depend on cytoskeleton architecture are critical to the mechanotransduction process, and have great potential for cancer diagnosis and therapy. In this study, the morphological and mechanical properties of typical osteosarcoma microenvironment cells, including mesenchymal stem cells (MSC), normal human osteoblast cells (NHOst) and osteosarcoma cells (MG-63), were compared using atomic force microscopy (AFM). The MG-63 cells were smaller and thicker than the MSC and NHOst cells. The membrane roughness of MG-63 cells was higher than that of MSC and NHOst cells. The MG-63 cells had lower stiffness than their normal counterparts due to their reduced organization of the cytoskeleton structure. The cell stiffness influenced the mechanotransduction. The MG-63 cells had a lower percentage of nuclear YAP/TAZ compared with the MSC and NHOst cells. The F-actin assembly was disrupted by the cytochalasin D (cyto D) treatment used to investigate its influence on mechanotransduction. Disruption of the cytoskeleton leaded to a decrease of the cell stiffness, and reduced the nuclear YAP/TAZ percentage, indicating its inhibition in the cell mechanotransduction process. This study would shed light on the development of a novel cancer diagnosis strategy and would contribute to reveal the relationship between the cytoskeleton structure and the cell mechanical properties.

Journal

  • Analytical Sciences

    Analytical Sciences 32(11), 1177-1182, 2016

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130005278005
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    0910-6340
  • NDL Article ID
    027713970
  • NDL Call No.
    Z54-F482
  • Data Source
    NDL  IR  J-STAGE 
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