Combinational Effects of Polymer Viscoelasticity and Immobilized Peptides on Cell Adhesion to Cell-selective Scaffolds

• KURIMOTO Rio

  Graduate School of Pure and Applied Sciences, University of Tsukuba International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS)

• KANIE Kei

  Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University

• UTO Koichiro

  International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS)

• KAWAI Shun

  Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University

• HARA Mitsuo

  Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University

• NAGANO Shusaku

  Nagoya University Venture Business Laboratory, Nagoya University

• NARITA Yuji

  Department of Cardiac Surgery, Nagoya University Graduate School of Medicine

• HONDA Hiroyuki

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• NAITO Masanobu

  Graduate School of Pure and Applied Sciences, University of Tsukuba Structural Materials Unit, Research Center for Strategic Materials, NIMS

• EBARA Mitsuhiro

  Graduate School of Pure and Applied Sciences, University of Tsukuba International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS) Graduate School of Industrial Science and Technology, Tokyo University of Science

• KATO Ryuji

  Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University

Abstract

Immobilization of functional peptides on polymer material is necessary to produce cell-selective scaffolds. However, the expected effects of peptide immobilization differ considerably according to the properties of selected polymers. To understand such combinational effects of peptides and polymers, varieties of scaffolds including a combination of six types of poly(ε-caprolactone-co-D,L-lactide) and four types of cell-selective adhesion peptides were fabricated and compared. On each scaffold, the scaffold properties (i.e. mechanical) and their biological functions (i.e. fibroblast-/endothelial cell-/smooth muscle cell-selective adhesion) were measured and compared. The results showed that the cell adhesion performances of the peptides were considerably enhanced or inhibited by the combination of peptide and polymer properties. In the present study, we illustrated the combinational property effects of peptides and polymers using multi-parametric analyses. We provided an example of determining the best scaffold performance for tissue-engineered medical devices based on quantitative data-driven analyses.

Journal

• Analytical Sciences

  Analytical Sciences 32 (11), 1195-1202, 2016

  The Japan Society for Analytical Chemistry

Keywords

• Biodegradable polymer
• cell adhesion peptide
• physicochemical property
• viscoelastic property
• cell-selective adhesion

Details

• CRID: 1390001204261588352
• NII Article ID: 130005278008
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.32.1195
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 027714013
• PubMed: 27829625
• Web Site: http://id.ndl.go.jp/bib/027714013
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027714013
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.32.1195.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.32.1195/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/32/11/32_1195/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2017176359
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed