A loss-of-function mutation in the <i>DWARF4</i>/<i>PETANKO5</i> gene enhances the late-flowering and semi-dwarf phenotypes of the <i>Arabidopsis</i> clock mutant <i>lhy-12;cca1-101</i> under continuous light without affecting <i>FLC</i> expression

• Suzuki Syunji

  Gene Research Center, University of Tsukuba

• Miyata Kana

  Gene Research Center, University of Tsukuba Department of Natural Sciences, International Christian University

• Hara Miyuki

  Gene Research Center, University of Tsukuba Department of Natural Sciences, International Christian University

• Niinuma Kanae

  Gene Research Center, University of Tsukuba

• Tsukaya Hirokazu

  Department of Biological Sciences, Graduate School of Science, The University of Tokyo Bio-Next Project, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences

• Takase Masahide

  Department of Biological Sciences, Graduate School of Science, The University of Tokyo

• Hayama Ryosuke

  Department of Natural Sciences, International Christian University

• Mizoguchi Tsuyoshi

  Gene Research Center, University of Tsukuba Department of Natural Sciences, International Christian University

Bibliographic Information

Other Title

• A loss-of-function mutation in the DWARF4/PETANKO5 gene enhances the late-flowering and semi-dwarf phenotypes of the Arabidopsis clock mutant lhy-12;cca1-101 under continuous light without affecting FLC expression

Abstract

The circadian clock plays important roles in the control of photoperiodic flowering in Arabidopsis. Mutations in the LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) genes (lhy;cca1) accelerate flowering under short days, whereas lhy;cca1 delays flowering under continuous light (LL). The lhy;cca1 mutant also exhibits short hypocotyls and petioles under LL. However, the molecular mechanisms underlying the regulation of both flowering time and organ lengths in the LHY/CCA1-dependent pathway are not fully understood. To address these questions, we performed EMS mutagenesis of the lhy-12;cca1-101 line and screened for mutations that enhance the lhy;cca1 phenotypes under LL. In this screen, we identified a novel allele of dwarf4 (dwf4) and named it petanko 5 (pta5). A similar level of enhancement of the delay in flowering was observed in these two dwf4 mutants when combined with the lhy;cca1 mutations. The lhy;cca1 and dwf4 mutations did not significantly affect the expression level of the floral repressor gene FLC under LL. Our results suggest that a defect in brassinosteroid (BR) signaling delayed flowering independent of the FLC expression level, at least in plants with the lhy;cca1 mutation grown under LL. The dwf4/pta5 mutation did not enhance the late-flowering phenotype of plants overexpressing SVP under LL, suggesting that SVP and BR function in a common pathway that controls flowering time. Our results suggest that the lhy;cca1 mutant exhibits delayed flowering due to both the BR signaling-dependent and -independent pathways under LL.

Journal

• Plant Biotechnology

  Plant Biotechnology 33 (4), 315-321, 2016

  Japanese Society for Plant Biotechnology

Keywords

• brassinosteroid
• CCA1
• DWF4
• flowering time
• LHY

Details

• CRID: 1390001204329504000
• NII Article ID: 130005281084
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.16.0601a
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 027729161
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027729161
• Web Site: https://www.jstage.jst.go.jp/article/plantbiotechnology/33/4/33_16.0601a/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed