Quinone-mediated induction of cytochrome P450 1A1 in HepG2 cells through increased interaction of aryl hydrocarbon receptor with aryl hydrocarbon receptor nuclear translocator

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Author(s)

Abstract

<p>While it has long been believed that benzenes and naphthalenes are unable to activate the aryl hydrocarbon receptor (AhR) because they are poor ligands, we recently reported that these quinoid metabolites upregulated cytochrome P450 1A1 (CYP1A1) in Hepa1c1c7 cells (Abiko<i> et al.</i>, 2015). In the current study, AhR activation, measured with a bioluminescence-based cell free assay, was induced by 1,2-naphthoquinone (1,2-NQ), a metabolite of naphthalene. Consistent with this, 1,4-benzoquinone (1,4-BQ), <i>tert</i>-butyl-1,4-BQ, and 1,4-NQ, as well as 1,2-NQ, all electrophilic mono- and bi-cyclic quinones, upregulated CYP1A1 mRNA and protein in HepG2 cells, whereas their parent aromatic hydrocarbons had little effect. Furthermore, immunofluorescence analysis confirmed that these quinones enhanced translocation of AhR to the nucleus.</p>

Journal

  • The Journal of Toxicological Sciences

    The Journal of Toxicological Sciences 41(6), 775-781, 2016

    The Japanese Society of Toxicology

Codes

  • NII Article ID (NAID)
    130005281972
  • NII NACSIS-CAT ID (NCID)
    AN00002808
  • Text Lang
    ENG
  • ISSN
    0388-1350
  • NDL Article ID
    027787676
  • NDL Call No.
    Z19-1022
  • Data Source
    NDL  J-STAGE 
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