Expression of CysLT2 receptors in asthma lung, and their possible role in bronchoconstriction

• Sekioka Tomohiko

  Discovery Research Laboratories II, Department of Biology & Pharmacology, Ono Pharmaceutical Co., Ltd Department of Pharmacology, Kyoto Pharmaceutical University

• Kadode Michiaki

  Discovery Research Laboratories II, Department of Biology & Pharmacology, Ono Pharmaceutical Co., Ltd Department of Pharmacology, Kyoto Pharmaceutical University

• Fujii Masanori

  Department of Pharmacology, Kyoto Pharmaceutical University

• Kawabata Kazuhito

  Discovery Research Laboratories II, Department of Biology & Pharmacology, Ono Pharmaceutical Co., Ltd

• Abe Takashi

  Department of Pneumology, Ogaki Municipal Hospital

• Horiba Michiaki

  Department of Pneumology, Ogaki Municipal Hospital

• Kohno Shigekatsu

  Department of Pharmacology, Kyoto Pharmaceutical University

• Nabe Takeshi

  Laboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University Department of Pharmacology, Kyoto Pharmaceutical University

書誌事項

タイトル別名

• Expression of CysLT₂ receptors in asthma lung, and their possible role in bronchoconstriction

抄録

Background: The expression and functional role of CysLT₂ receptors in asthma have not been clarified. In this study, we evaluated CysLT₂ receptors expression, and effects of CysLT₂-and CysLT_{1 ⁄ 2}-receptor an- tagonists on antigen-induced bronchoconstriction using isolated lung tissues from both asthma and non- asthma subjects. Methods: CysLT₁ and CysLT₂ receptors expression in asthma and non-asthma lung tissue preparations was examined in immunohistochemistry experiments, and their functional roles in antigen-induced bronchoconstriction were assessed using ONO-6950, a dual CysLT_{1 ⁄ 2}-receptor antagonist, montelukast, a CysLT₁ receptor antagonist, and BayCysLT₂RA, a CysLT₂ receptor-specific antagonist. Results: CysLT₁ receptors were expressed on the bronchial smooth muscle and epithelium, and on alveolar leukocytes in 5 in 5 non-asthma subjects and 2 in 2 asthma subjects. On the other hand, although degrees of CysLT₂ receptors expression were variable among the 5 non-asthma subjects, the expression in the asthma lung was detected on bronchial smooth muscle, epithelium and alveolar leu- kocytes in 2 in 2 asthma subjects. In the non-asthma specimens, antagonism of CysLT₂ receptors did not affect antigen-induced bronchial contractions, even after pretreatment with the CysLT₁-receptor specific antagonist, montelukast. However, in the bronchus isolated from one of the 2 asthma subjects, antag- onism of CysLT₂ receptors suppressed contractions, and dual antagonism of CysLT₁ and CysLT₂ receptors resulted in additive inhibitory effect on anaphylactic contractions. Conclusions: CysLT₂ receptors were expressed in lung specimens isolated from asthma subjects. Acti- vation of CysLT₂ receptors may contribute to antigen-induced bronchoconstriction in certain asthma population.

収録刊行物

• Allergology International

  Allergology International 64 (4), 351-358, 2015

  一般社団法人日本アレルギー学会

キーワード

• Airway smooth muscle, Anti-asthma drugs, CysLT receptors, Cysteinyl leukotrienes, Leukotriene receptor antagonist

詳細情報

• CRID: 1390001204631707008
• NII論文ID: 130005290332
• DOI: 10.1016/j.alit.2015.04.008
• ISSN: 14401592
• ISSN: 13238930
• Web Site: https://api.elsevier.com/content/article/PII:S1323893015001069?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S1323893015001069?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2016324497
• 本文言語コード: en
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