Presensitization to Ascaris antigens promotes induction of mite-specific IgE upon mite antigen inhalation in mice

• Suzuki Mayu

  Atopy (Allergy) Research Center, Juntendo University Graduate School of Medicine Department of Materials and Biological Sciences, Faculty of Science, Japan Women's University

• Hara Mutsuko

  Atopy (Allergy) Research Center, Juntendo University Graduate School of Medicine

• Ichikawa Saori

  Department of Materials and Biological Sciences, Faculty of Science, Japan Women's University

• Kamijo Seiji

  Atopy (Allergy) Research Center, Juntendo University Graduate School of Medicine

• Nakazawa Takuya

  Clinical Research Center for Allergy and Rheumatology, National Hospital Organization Sagamihara National Hospital Department of Rheumatology, Allergy, and Clinical Immunology, National Hospital Organization Chiba-East National Hospital

• Hatanaka Hideki

  National Bioscience Database Center, Japan Science and Technology Agency

• Akiyama Kazuo

  Clinical Research Center for Allergy and Rheumatology, National Hospital Organization Sagamihara National Hospital

• Ogawa Hideoki

  Atopy (Allergy) Research Center, Juntendo University Graduate School of Medicine

• Okumura Ko

  Atopy (Allergy) Research Center, Juntendo University Graduate School of Medicine

• Takai Toshiro

  Atopy (Allergy) Research Center, Juntendo University Graduate School of Medicine

抄録

Background: Patients with house dust mite (HDM) allergy or Ascariasis produce serum IgE specific to the antigens of HDM or nematode Ascaris, respectively. Although human IgE cross-reactivity has been reported between HDM and Ascaris antigens, it remains unclear whether it contributes to the pathogenesis of allergic diseases. We herein investigated the induction of cross-reactive antibodies and T cells in mice and effects of airway exposure to HDM antigens after preimmunization with Ascaris antigens. Methods: Mice were intraperitoneally immunized with HDM or Ascaris antigens with Alum, followed by the intranasal administration of HDM antigens. Serum antigen-specific IgE and IgG were measured by ELISA. Cytokine release in splenocytes from Ascaris-immunized mice upon in vitro restimulation with HDM antigens were measured by ELISA. Results: Immunization with Ascaris or HDM antigens induced cross-reactive IgG1. Splenocytes from Ascaris-immunized mice released IL-5 and IL-13 in response to the restimulation with HDM antigens.Subsequent airway exposure to HDM antigens promoted the induction of HDM-specific IgE and upregulation of HDM-specific IgG1 in Ascaris-immunized mice, whereas these responses were not detected or smaller without the Ascaris presensitization. Conclusions: We demonstrated that the immunization of naive mice with Ascaris antigens induced production of antibodies and differentiation of Th2 cells, which were cross-reactive to HDM antigens, and accelerated induction of serum HDM-specific IgE upon subsequent airway exposure to HDM antigens in mice. These results suggest that sensitization to HDM towards IgE-mediated allergic diseases is faster in individuals with a previous history of Ascaris infection than in those without presensitization to Ascaris.

収録刊行物

• Allergology International

  Allergology International 65 (1), 44-51, 2016

  一般社団法人日本アレルギー学会

キーワード

• Antibody; Ascaris; Cross-reactivity; House dust mite; Th2

詳細情報

• CRID: 1390282679610328064
• NII論文ID: 130005290333
• DOI: 10.1016/j.alit.2015.07.003
• ISSN: 14401592
• ISSN: 13238930
• Web Site: https://api.elsevier.com/content/article/PII:S1323893015001525?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S1323893015001525?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2016261857
• 本文言語コード: en
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