The expression of miR-124 increases in aged skin to cause cell senescence and it decreases in squamous cell carcinoma
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- Harada Miho
- Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
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- Jinnin Masatoshi
- Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
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- Wang Zhongzhi
- Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
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- Hirano Ayaka
- Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
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- Tomizawa Yukiko
- Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
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- Kira Tomomi
- Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
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- Igata Toshikatsu
- Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
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- Masuguchi Shinichi
- Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
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- Fukushima Satoshi
- Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
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- Ihn Hironobu
- Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
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Abstract
<p>Skin senescence is induced by various factors including intrinsic aging and extrinsic aging. The current study compared the expression of microRNAs in young facial skin and senescent facial skin, and this study identified skin aging-related microRNAs. According to the results from a microRNA PCR Array, miR-124 was the microRNA that increased the most in senescent skin compared to young skin. Real-time PCR with a greater number of samples indicated that the increase in miR-124 levels in senescent facial skin was statistically significant. In situ hybridization was performed, and results indicated that the signal for miR-124 was evident in keratinocytes of senescent skin but not in those of young skin. The morphology of cultured normal human epidermal keratinocytes (NHEKs) transfected with a miR-124 mimic changed to an enlarged and irregular shape. In addition, the number of NHEKs positive for senescence-associated β-galactosidase (SA-β-gal) increased significantly as a result of the overexpression of the miR-124 mimic. The expression of miR-124 increased in UVB-irradiated NHEKs compared to controls in a dose-dependent manner. Expression of miR-124 in A431, a human cutaneous squamous cell carcinoma (SCC) cell line, decreased significantly compared to that in NHEKs. Forced overexpression of miR-124 as a result of the transfection of a miR-124 mimic in A431 resulted in the significant suppression of the proportion of cancer cells. The current results indicated that miR-124 increases as a result of cell senescence and that it decreases during tumorigenesis. The effect of supplementation of miR-124 in an SCC cell line suggests that senescence induction therapy with microRNA may be a new therapeutic approach for treatment of SCC.</p>
Journal
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- BioScience Trends
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BioScience Trends 10 (6), 454-459, 2016
International Research and Cooperation Association for Bio & Socio-Sciences Advancement