P-54 担子菌由来α-L-アラビノフラノシダーゼの機能解析  [in Japanese] P-54 Characterization of glycoside hydrolase family 51 α-L-arabinofuranosidases from basidiomycetes  [in Japanese]

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Abstract

<p>Two α-L-arabinofuranosidase genes classified in Glycoside Hydrolase (GH) family 51 from the basidiomycetes Phanerochaete chrysosporium and Flammulina velutipes were cloned, and the recombinant enzymes expressed in the yeast Pichia pastoris were characterized. When p-nitrophenyl-α-L-arabinofuranoside was used as a substrate, two enzymes showed acidic pH optima at pH 3. The two enzymes released L-arabinose from rye arabinoxylan, wheat arabinoxylan, sugar beet arabinan and debranched arabinan, but not from arabinogalactan.</p>

Journal

  • Proceedings of the Conference on Biomass Science

    Proceedings of the Conference on Biomass Science 12(0), 157-158, 2017

    The Japan Institute of Energy

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