Whole Genome Sequencing-Based Molecular Epidemiologic Analysis of Autochthonous Dengue Virus Type 1 Strains Circulating in Japan in 2014

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  • Tajima Shigeru
    Department of Virology I, National Institute of Infectious Diseases
  • Nakayama Eri
    Department of Virology I, National Institute of Infectious Diseases
  • Kotaki Akira
    Department of Virology I, National Institute of Infectious Diseases
  • Moi Meng Ling
    Department of Virology I, National Institute of Infectious Diseases
  • Ikeda Makiko
    Department of Virology I, National Institute of Infectious Diseases
  • Yagasaki Kazumi
    Department of Virology I, National Institute of Infectious Diseases
  • Saito Yuka
    Department of Virology I, National Institute of Infectious Diseases
  • Shibasaki Ken-ichi
    Department of Virology I, National Institute of Infectious Diseases
  • Saijo Masayuki
    Department of Virology I, National Institute of Infectious Diseases
  • Takasaki Tomohiko
    Department of Virology I, National Institute of Infectious Diseases

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Abstract

Cases of autochthonous infections of dengue virus type 1 (DENV-1) were detected in Japan after a 70-year period devoid of dengue outbreaks. We previously showed that E gene sequences are identical in 11 of the 12 DENV-1 strains autochthonous to Japan. However, the E sequence represents only 14% of the DENV-1 genome. In the present study, we have sequenced the entire genome of 6 autochthonous DENV-1 strains that were isolated from patients during the 2014 outbreak. Sequencing of 5 Yoyogi group strains with identical E sequences and 1 Shizuoka strain with a different E sequence revealed that the first Yoyogi group strain differed from the Shizuoka strain by 18 amino acid residues. Furthermore, 2 Yoyogi group strains had different genomic sequences while the other 3 had identical genomes. Phylogenetic analyses indicated that the Hyogo strain, a Yoyogi group strain, was the first to diverge from the other 4 Yoyogi group strains. The E gene sequence of the Yoyogi group strains exhibits the highest homology to those of the strains isolated in Malaysia and Singapore between 2013 and 2014. The patient infected with the Hyogo strain visited Malaysia before the onset of dengue fever, suggesting that this was a case of dengue infection imported from Malaysia.

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 70 (1), 45-49, 2017

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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