Oral administration of heat-killed <i>Lactobacillus kunkeei</i> YB38 improves murine influenza pneumonia by enhancing IgA production

• ASAMA Takashi

  Institute for Bee Products and Health Science, Yamada Bee Company, Inc., Okayama 708-0393, Japan

• UEMATSU Takayuki

  Biomedical Laboratory, Division of Biomedical Research, Kitasato University Medical Center, Saitama 364-8501, Japan

• KOBAYASHI Noritada

  Biomedical Laboratory, Division of Biomedical Research, Kitasato University Medical Center, Saitama 364-8501, Japan

• TATEFUJI Tomoki

  Institute for Bee Products and Health Science, Yamada Bee Company, Inc., Okayama 708-0393, Japan

• HASHIMOTO Ken

  Institute for Bee Products and Health Science, Yamada Bee Company, Inc., Okayama 708-0393, Japan

Abstract

Influenza is one of the important respiratory tract infections that require special attention for maintaining health and hygiene. The removal of influenza virus (IFV) by secretory IgA produced by the respiratory epithelium has been reported to be a critical host defense mechanism. Therefore, we isolated Lactobacillus kunkeei YB38 (YB38), the promoter of the salivary IgA secretion in humans, from honeybee pollen and studied the effect of heat-killed YB38 treatment for preventing IFV infection in a mouse model. Female BALB/c mice received YB38 orally for 21 consecutive days and were then inoculated nasally with IFV. The YB38-treated group with a daily dose of 100 mg/kg showed an increased survival rate after IFV infection relative to the control. IgA secretion in the respiratory epithelium in the YB38-treated group (100 mg/kg) was significantly increased after 6 days of infection, while IL-6 production in the same respiratory site and the number of cells infiltrating into alveoli were significantly decreased. Moreover, lung tissue damage that appeared after IFV infection was reduced. These results suggested that the YB38 dose induced early and local IgA secretion at the infection site, inhibited persistent IFV infection, and prevented the infiltration of inflammatory immune cells or production of excessive IL-6, resulting in less damage to lung tissues.

Journal

• Bioscience of Microbiota, Food and Health

  Bioscience of Microbiota, Food and Health 36 (1), 1-9, 2017

  BMFH Press

Keywords

• influenza virus
• mice
• <i>Lactobacillus kunkeei</i> YB38
• immunoglobulin A

Details

• CRID: 1390282680386958976
• NII Article ID: 130005303592
• DOI: 10.12938/bmfh.16-010
• ISSN: 21863342
• Web Site: https://www.jstage.jst.go.jp/article/bmfh/36/1/36_16-010/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2017326445
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed