Effects of sublingual immunotherapy in a murine asthma model sensitized by intranasal administration of house dust mite extracts

• Shima Kenjiro

  Department of Respiratory Medicine and Infectious Diseases, Niigata University Graduate School of Medical and Dental Sciences

• Koya Toshiyuki

  Department of Respiratory Medicine and Infectious Diseases, Niigata University Graduate School of Medical and Dental Sciences

• Tsukioka Keisuke

  Department of Respiratory Medicine and Infectious Diseases, Niigata University Graduate School of Medical and Dental Sciences

• Sakagami Takuro

  Department of Respiratory Medicine and Infectious Diseases, Niigata University Graduate School of Medical and Dental Sciences

• Hasegawa Takashi

  Department of General Medicine, Niigata University Medical and Dental Hospital

• Fukano Chiharu

  Research Laboratory, Torii Pharmaceutical Co., Ltd.

• Ohashi-Doi Katsuyo

  Research Laboratory, Torii Pharmaceutical Co., Ltd.

• Watanabe Satoshi

  Department of Respiratory Medicine and Infectious Diseases, Niigata University Graduate School of Medical and Dental Sciences

• Suzuki Eiichi

  Department of General Medicine, Niigata University Medical and Dental Hospital

• Kikuchi Toshiaki

  Department of Respiratory Medicine and Infectious Diseases, Niigata University Graduate School of Medical and Dental Sciences

抄録

Background: Sublingual immunotherapy (SLIT) has received attention as a method for allergen immu-notherapy. However, the mechanism of SLIT has not yet been fully investigated. Therefore, we evaluated the effects of SLIT in a murine asthma model, sensitized by intranasal administration of house dust mite (HDM) extracts. Methods: Female BALB/c mice were intranasally exposed to HDM for either 3 or 5 weeks (5 consecutive days per week). Mice were administered either low-dose (0.5 mg/day) or high-dose (5 mg/day) sub-lingual HDM extracts for 2 weeks, followed by an additional week of intranasal exposure. Airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid (BALF) cell count, cytokine levels in the BALF and lymph node cell culture supernatants, and allergen-specific antibodies were measured. Lung his-tology was also investigated. Results: In mice sensitized for 5 weeks, high-dose SLIT ameliorated AHR, airway eosinophilia and goblet cell metaplasia. In mice sensitized for 3 weeks, even low dose SLIT ameliorated AHR and airway eosinophilia. Th2 cytokine levels in culture supernatants of submandibular lymph node cells in high-dose SLIT mice decreased, whereas IL-10 levels increased. Total IgA in BALF increased in mice sensi-tized for 3 or 5 weeks, and high-dose SLIT also increased allergen-specific IgG2a in mice sensitized for 5 weeks. Conclusions: These data suggest that earlier induction of SLIT in HDM-sensitized mice provides superior suppression of AHR and goblet cell metaplasia. The modulation of allergen specific IgG2a and local IgA might play a role in the amelioration of AHR and airway inflammation.

収録刊行物

• Allergology International

  Allergology International 66 (1), 89-96, 2017

  一般社団法人日本アレルギー学会

キーワード

• Airway hyperresponsiveness
• Animal model
• Bronchial asthma
• House dust mites
• Sublingual immunotherapy

詳細情報

• CRID: 1390282679609840384
• NII論文ID: 130005308448
• DOI: 10.1016/j.alit.2016.05.012
• ISSN: 14401592
• ISSN: 13238930
• Web Site: https://api.elsevier.com/content/article/PII:S1323893016300831?httpAccept=text/plain
• Web Site: https://api.elsevier.com/content/article/PII:S1323893016300831?httpAccept=text/xml
• Web Site: https://search.jamas.or.jp/link/ui/2017241195
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
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