Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium
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- TOKITA Reiko
- Department of Clinical Pathophysiology, Tokyo Dental College
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- NAKAJIMA Kei
- Department of Clinical Pathophysiology, Tokyo Dental College Oral Health Science Center, Tokyo Dental College
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- INOUE Kenji
- Department of Clinical Pathophysiology, Tokyo Dental College Oral Health Science Center, Tokyo Dental College
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- AL-WAHABI Akram
- Department of Clinical Pathophysiology, Tokyo Dental College
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- SER-OD Tungalag
- Department of Clinical Pathophysiology, Tokyo Dental College
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- MATSUZAKA Kenichi
- Department of Clinical Pathophysiology, Tokyo Dental College Oral Health Science Center, Tokyo Dental College
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- INOUE Takashi
- Department of Clinical Pathophysiology, Tokyo Dental College Oral Health Science Center, Tokyo Dental College
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Abstract
<p>In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. The sum of these findings suggests that PLGA does not disturb the osteogenesis of iPS cells.</p>
Journal
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- Dental Materials Journal
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Dental Materials Journal 36 (1), 103-110, 2017
The Japanese Society for Dental Materials and Devices
