Preparation of a Surface-functionalized Power-free PDMS Microchip for MicroRNA Detection Utilizing Electron Beam-induced Graft Polymerization

• ISHIHARA Ryo

  Department of Materials Science and Technology, Tokyo University of Science

• UCHINO Yoshitaka

  Department of Materials Science and Technology, Tokyo University of Science

• HOSOKAWA Kazuo

  Bioengineering Laboratory, RIKEN

• MAEDA Mizuo

  Bioengineering Laboratory, RIKEN

• KIKUCHI Akihiko

  Department of Materials Science and Technology, Tokyo University of Science

Abstract

We propose an easy microchannel surface functionalization method for a poly(dimethylsiloxane) (PDMS) microchip that utilizes electron beam-induced graft polymerization (EIGP) as a platform for microchip-based biomarker analysis. Unlike other grafting techniques, EIGP enables rapid surface modification of PDMS without initiator immobilization. The grafted microchip is preservable, and can be easily functionalized for versatile applications. In this study, the surface-functionalized power-free microchip (SF-PF microchip) was used for the detection of microRNA (miRNA), which is a biomarker for many serious diseases. The EIGP enables high-density three-dimensional probe DNA immobilization, resulting in rapid and sensitive miRNA detection on the portable SF-PF microchip. The limit of detection was 0.8 pM, the required sample volume was 0.5 μL, and the analysis time was 15 min. The SF-PF microchip will be a versatile platform for microchip-based point-of-care diagnosis.

Journal

• Analytical Sciences

  Analytical Sciences 33 (2), 197-202, 2017

  The Japan Society for Analytical Chemistry

Keywords

• Electron beam-induced graft polymerization
• surface-functionalized power-free microchip
• microRNA
• point-of-care diagnosis

Details

• CRID: 1390282679237463680
• NII Article ID: 130005316213
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.33.197
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 027968950
• PubMed: 28190840
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027968950
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.33.197.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.33.197/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/33/2/33_197/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed