Hypoxia-inducible factor 1 mediates hypoxia-enhanced synthesis of progesterone during luteinization of granulosa cells

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Author(s)

    • FADHILLAH FADHILLAH
    • Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Sciences, Okayama University, Okayama 700-8530, Japan
    • YOSHIOKA Shin YOSHIOKA Shin
    • Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Sciences, Okayama University, Okayama 700-8530, Japan
    • NISHIMURA Ryo NISHIMURA Ryo
    • Laboratory of Theriogenology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8550, Japan
    • YAMAMOTO Yuki YAMAMOTO Yuki
    • Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Sciences, Okayama University, Okayama 700-8530, Japan
    • KIMURA Koji KIMURA Koji
    • Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Sciences, Okayama University, Okayama 700-8530, Japan
    • OKUDA Kiyoshi OKUDA Kiyoshi
    • Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Sciences, Okayama University, Okayama 700-8530, Japan|Obihiro University of Agriculture and Veterinary Medicine, Inada Nishi 2-11, Obihiro 080-8555, Japan

Abstract

<p> Hypoxia has been suggested to enhance progesterone (P4) synthesis in luteinizing granulosa cells (GCs), but the mechanism is unclear. The present study was designed to test the hypothesis that the hypoxia-induced increase in P4 synthesis during luteinization in bovine GCs is mediated by hypoxia-inducible factor 1 (HIF-1). GCs obtained from small antral follicles were cultured with 2 µg/ml insulin in combination with 10 µM forskolin for 24 h as a model of luteinizing GCs. To examine the influence of HIF-1 on P4 synthesis, we determined the effect of changes in protein expression of the α-subunit of HIF-1 (HIF1A) on P4 production and on the expression levels of StAR, P450scc, and 3β-HSD. CoCl<sub>2</sub> (100 µM), a hypoxia-mimicking chemical, increased HIF-1α protein expression in luteinizing GCs. After the upregulation of HIF-1α, we observed an increase in P4 production and in the gene and protein expression levels of StAR in CoCl<sub>2</sub>-treated luteinizing GCs. In contrast, CoCl<sub>2</sub> did not affect the expression of either P450scc or 3β-HSD. Echinomycin, a small-molecule inhibitor of HIF-1's DNA-binding activity, attenuated the effects of CoCl<sub>2</sub> and of low oxygen tension (10% O<sub>2</sub>) on P4 production and StAR expression in luteinizing GCs. Overall, these findings suggest that HIF-1 is one of the factors that upregulate P4 in GCs during luteinization.</p>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 63(1), 75-85, 2017

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

Codes

  • NII Article ID (NAID)
    130005332285
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • ISSN
    0916-8818
  • NDL Article ID
    027985605
  • NDL Call No.
    Z54-H305
  • Data Source
    NDL  J-STAGE 
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