Involvement of DNA methylation in regulating rat Prop1 gene expression during pituitary organogenesis
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- NISHIHARA Hiroto
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
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- YOSHIDA Saishu
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan Institute for Endocrinology, Meiji University, Kanagawa 214-8571, Japan
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- KANNO Naoko
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
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- NISHIMURA Naoto
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
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- UEHARU Hiroki
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
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- OHGANE Jun
- Institute for Endocrinology, Meiji University, Kanagawa 214-8571, Japan School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
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- KATO Takako
- Institute for Endocrinology, Meiji University, Kanagawa 214-8571, Japan
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- KATO Yukio
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan Institute for Endocrinology, Meiji University, Kanagawa 214-8571, Japan School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
書誌事項
- タイトル別名
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- Involvement of DNA methylation in regulating rat <i>Prop1</i> gene expression during pituitary organogenesis
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抄録
<p> PROP1 is a pituitary specific transcription factor that plays a crucial role in pituitary organogenesis. The Prop1 shows varied expression patterns that promptly emerge and then fade during the early embryonic period. However, the regulatory mechanisms governing Prop1 expression remain unclear. Here, we investigated whether Prop1 was under epigenetic regulation by DNA methylation. Bisulfite sequencing was performed on DNA obtained from the pituitary glands and livers of rats on embryonic days (E) 13.5 and E14.5, and postnatal days (P) 4 and P30. The methylation of CpG sites in seven regions from 3-kb upstream of the Prop1 transcription start site through to its second intron were examined. Certain differences in CpG-methylation levels were observed in Region-1 (–2772 b to –2355 b), Region-4 (–198 b to +286 b), Region-5 (+671 b to +990 b), and Region-6 (+1113 b to +1273 b) based on comparisons between pituitary and liver DNA on E13.5. DNA methylation in pituitary glands on E14.5, P4, and P30 was generally similar to that observed in in the pituitary gland on E13.5, whereas the anterior and intermediate lobes of the pituitary gland on P4 and P30 showed only small differences. These results indicate that Prop1 is under regulation by CpG methylation during the early period of pituitary primordium development around E13.5.</p>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 63 (1), 37-44, 2017
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681314894464
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- NII論文ID
- 130005332293
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 027985550
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- PubMed
- 27773885
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可