Effect of Biological Rhythm Synchronous Factor Melatonin on Structure, Composition, and Calcification of Dentin and Odontoblasts

• MISHIMA Hiroyuki

  Department of Medical Hygiene, Kochi Gakuen College, Kochi, Japan

• TAKEUCHI Shohei

  Department of Clinical laboratory, Mizushima Community Hospital, Kurashiki, Japan

• TANABE Saki

  Applied Life Science Course, Kochi Gakuen College, Kochi, Japan

• HATTORI Atsuhiko

  Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Japan

• SUZUKI Nobuo

  Institute of Nature and Environmental Technology, Kanazawa University, Housu-gun, Japan

• KAKEI Mitsuo

  Division of Oral Anatomy, Meikai University School of Dentistry, Sakado, Japan

• MATUMOTO Takashi

  Department of Laboratory Diagnosis, University Hospital, Nihon University School of Dentistry at Matsudo, Matsudo, Japan

• IKEGAME Mika

  Department of Oral Morphology, Science of Functional Recovery and Reconstruction, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Advanced Research Center for Oral and Craniofacial Sciences, Okayama University, Okayama, Japan

• TAKECHI Kazuhiko

  Department of Medical Hygiene, Kochi Gakuen College, Kochi, Japan

• MIAKE Yasuo

  Department of Histology and Developmental Biology, Tokyo Dental College, Tokyo, Japan

Bibliographic Information

Other Title

• 生体リズム同調因子メラトニンが象牙芽細胞，象牙質の構造及び石灰化機構に及ぼす影響

Abstract

The purpose of the present study is to examine the influence of melatonin on the structure and calcification of both dentin and odontoblasts. The size of calcospherites in predentin increased in proportion to the concentration of melatonin administered. On the melatonin treated groups, the von Korff's fibers were clear and long. The number of odontoblasts tended to increase. The expression of melatonin receptor MT2 was observed in the odontoblasts of the control group. In the melatonin treated groups, a strong expression of MT2 was observed in the odontoblasts. By MELDI TOF-MS analysis, a peak of around 850 m/z was observed in dentin. This peak increased in proportion to the amount of melatonin. It is considered that melatonin activates the odontoblasts and promotes the formation of von Korff's fibers, collagen fiber secretion and calcification of dentin.

Journal

• Journal of the Japanese Association of Regenerative Dentistry

  Journal of the Japanese Association of Regenerative Dentistry 14 (1), 5-17, 2016

  Japanese Association of Regenerative Dentistry

Keywords

• dentin
• odontoblast
• melatonin
• melatonin receptor
• von Korff's fiber

Details

• CRID: 1390001205227667712
• NII Article ID: 130005395190
• DOI: 10.11223/jard.14.5
• ISSN: 18800815
• ISSN: 13489615
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed