Development of a Cartridge-type Cell Culture Device Combined with Poly(N-isopropylacrylamide) Coated Substrates

• Kinoshita Yumiko

  Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology

• Park Jongho

  Laboratory of Future Interdisciplinary Research and Science Technology, Tokyo Institute of Technology

• Yanagida Yasuko

  Laboratory of Future Interdisciplinary Research and Science Technology, Tokyo Institute of Technology

• Hatsuzawa Takeshi

  Laboratory of Future Interdisciplinary Research and Science Technology, Tokyo Institute of Technology

Abstract

Cell subculture processes, such as medium exchange and cell detachment, are essential steps for maintaining cells healthy during performing experiments. Simplification of subculture processes would benefit cells' viability by reducing the number of steps performed by researchers, consequently minimizing cell stress. In particular, it is considered important to reduce the damage caused by the enzymatic treatment to detach adherent cells. We developed a new subculture device to replace conventional enzymatic treatments with the use of a thermoresponsive substrate in this work. Thermoresponsive substrates were prepared coating poly N-isopropylacrylamide with different concentrations onto glass substrates pre-coated with a silane-coupling agent. Prepared thermoresponsive substrates were evaluated by cell proliferation and detachment tests as well as the measurement of contact angles with respect to different pNIPAAm concentrations. Developed culture device was then evaluated by performing cell subculture, which results in achieving the cell proliferation and detachment rates over 80%. It was confirmed the use of a cartridge-type cell culture device allows faster and easier cell detachment in subculture process. We finally confirmed that the feasibility of developed cell culture device for its potential applications in cell related researches.

Journal

• IEEJ Transactions on Sensors and Micromachines

  IEEJ Transactions on Sensors and Micromachines 137 (3), 78-83, 2017

  The Institute of Electrical Engineers of Japan

Keywords

• cartridge cell-culture device
• poly N-isopropylacrylamide
• thermoresponsive
• adherent animal cell
• subculture

Details

• CRID: 1390001204460060416
• NII Article ID: 130005398000
• NII Book ID: AN1052634X
• DOI: 10.1541/ieejsmas.137.78
• ISSN: 13475525
• ISSN: 13418939
• NDL BIB ID: 028034760
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028034760
• Web Site: https://www.jstage.jst.go.jp/article/ieejsmas/137/3/137_78/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed