Claudin-2 expression is upregulated in the ileum of diarrhea predominant irritable bowel syndrome patients

• Ishimoto Haruka

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

• Oshima Tadayuki

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

• Sei Hiroo

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

• Yamasaki Takahisa

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

• Kondo Takashi

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

• Tozawa Katsuyuki

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

• Tomita Toshihiko

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

• Ohda Yoshio

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

• Fukui Hirokazu

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

• Watari Jiro

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

• Miwa Hiroto

  Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine

抄録

Intestinal epithelial barrier function is impaired in irritable bowel syndrome patients. Claudins are highly expressed in cells with tight junctions and are involved in the intestinal epithelial barrier function. The expression pattern of tight junction proteins in diarrhea-predominant irritable bowel syndrome have not been fully elucidated. We therefore recruited 17 diarrhea-predominant irritable bowel syndrome patients and 20 healthy controls. The expression of the tight junction-related proteins was examined in the ileal, cecal, and rectal mucosa of diarrhea-predominant irritable bowel syndrome patients using real-time PCR and immunofluorescence. Claudin-2 expression was high in the ileum of diarrhea-predominant irritable bowel syndrome patients. Claudin-2 expression was the same in cecum and rectal mucosa of control and diarrhea-predominant irritable bowel syndrome patients. Similarly, the expression of clauidn-1, claudin-7, JAM-A, occludin, and ZO-1 in the ileal, cecal, and rectal mucosa did not change between control and diarrhea-predominant irritable bowel syndrome samples. Infiltration of eosinophil and mast cells in the mucosa of ileum, cecum and rectum was evaluated using immunohistochemical staining and was not affected by diarrhea-predominant irritable bowel syndrome. Claudin-2 was expressed on the apical side of villi and crypts of ileal mucosal epithelial cells. Clauidn-2 expression is upregulated in diarrhea-predominant irritable bowel syndrome patients and may contribute to the pathogenesis of this condition.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 60 (2), 146-150, 2017

  一般社団法人　日本酸化ストレス学会

キーワード

• claudins
• irritable bowel syndrome
• tight junction
• mast cell
• eosinophil

詳細情報

• CRID: 1390001204672913920
• NII論文ID: 130005398540
• DOI: 10.3164/jcbn.16-92
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://www.jstage.jst.go.jp/article/jcbn/60/2/60_16-92/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018000410
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可