<I>Candida albicans</I> Adheres to Chitin by Recognizing <I>N</I>-acetylglucosamine (GlcNAc)  [in Japanese]

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Author(s)

Abstract

The binding of <I>Candida albicans</I> cells to chitin was examined in a cell-binding assay. Microscopic observations indicated that both living and heat-killed <I>Candida</I> cells bound to chitin-coated substrates. <I>C. albicans</I> preferentially bound to chitin-coated plastic plates over chitosan-coated and uncoated plates. We prepared <sup>125</sup>I-labeled <I>Candida</I> cells for quantitative analysis of their binding to chitin. Heat-killed <sup>125</sup>I-labeled <I>Candida</I> cells bound to chitin-coated plates in a time-dependent manner until 1.5 hours after start of incubation at 4℃. The binding of <sup>125</sup>I-labeled <I>Candida</I> cells to chitin-coated plates was inhibited by adding unlabeled living or unlabeled heat-killed <I>Candida</I> cells. The binding of <I>Candida</I> to chitin was also reduced by addition of 25 mg/ml chitin or chitosan up to 10%. <I>N</I>-acetylglucosamine (GlcNAc), which is a constituent of chitin, inhibited binding of <I>Candida</I> to chitin in a dose-dependent manner between 12.5 and 200 mM. Glucosamine, which is a constituent of chitosan, showed no such inhibitory effect. These findings suggest that the binding of <I>Candida</I> to chitin may be mediated by recognition of GlcNAc.

Journal

  • Medical Mycology Journal

    Medical Mycology Journal 58(1), E15-E21, 2017

    The Japanese Society for Medical Mycology

Codes

  • NII Article ID (NAID)
    130005401911
  • NII NACSIS-CAT ID (NCID)
    AA12518136
  • Text Lang
    JPN
  • ISSN
    2185-6486
  • NDL Article ID
    027969713
  • NDL Call No.
    Z19-348
  • Data Source
    NDL  J-STAGE 
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