The fertilization ability and developmental competence of bovine oocytes grown <i>in vitro</i>

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Author(s)

    • MAKITA Miho
    • Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
    • UEDA Mayuko
    • Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
    • MIYANO Takashi
    • Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan

Abstract

<p><i>In vitro</i> growth culture systems for oocytes are being developed in several mammalian species. In these growth culture systems, <i>in vitro</i> grown oocytes usually have lower blastocyst formation than <i>in vivo</i> grown oocytes after <i>in vitro</i> fertilization. Furthermore, there have been a few reports that investigated the fertilization ability of <i>in vitro</i> grown oocytes in large animals. The purpose of this study was to investigate the fertilization process and developmental competence of bovine oocytes grown <i>in vitro</i>. Oocyte-granulosa cell complexes collected from bovine early antral follicles (0.4−0.7 mm in diameter) were cultured for growth with 17β-estradiol and androstenedione for 14 days and matured <i>in vitro</i>. These oocytes were then inseminated for 6 or 12 h, and further cultured for development up to 8 days <i>in vitro</i>. After growth culture, oocytes grew from 95 µm to around 120 µm and acquired maturation competence (79%). Although fertilization rates of <i>in vitro</i> grown oocytes were low after 6 h of insemination, 34% of <i>in vitro</i> grown oocytes fertilized normally after 12 h of insemination, having two polar bodies and two pronuclei with a sperm tail, and 22% of these oocytes developed into blastocysts after 8 days of culture. The fertilization and blastocyst formation rates were similar to those of <i>in vivo</i> grown oocytes. In addition, blastocyst cell numbers were also similar between <i>in vitro</i> and <i>in vivo</i> grown oocytes. In conclusion, <i>in vitro</i> grown bovine oocytes are similar to <i>in vivo</i> grown oocytes in fertilization ability and can develop into blastocysts.</p>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 62(4), 379-384, 2016

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

Codes

  • NII Article ID (NAID)
    130005406795
  • Text Lang
    ENG
  • ISSN
    0916-8818
  • Data Source
    J-STAGE 
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