Contribution of Lewis X Carbohydrate Structure to Neuropathogenic Murine Coronaviral Spread

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Author(s)

Abstract

Although Lewis X (Le<sup>x</sup>), a carbohydrate structure, is involved in innate immunity through cell-to-cell and pathogen recognition, its expression has not been observed in mouse monocytes/macrophages (Mo/Mas). The Mo/Mas that infiltrate the meninges after infection with the neuropathogenic murine coronavirus strain srr7 are an initial target of infection. Furthermore, higher inflammatory responses were observed in gene-manipulated mice lacking α1,3-fucosyltransferase 9, which determines the expression of the Le<sup>x</sup> structure, than in wild type mice after infection. We investigated Le<sup>x</sup> expression using CD11b-positive peritoneal exudate cells (PECs) and found that Le<sup>x</sup> is inducible in Mo/Mas after infection with srr7, especially in the syncytial cells during the late phase of infection. The number of syncytial cells was reduced after treatment of the infected PECs with anti-Le<sup>x</sup> antibody, during the late phase of infection. In addition, the antibody treatment induced a marked reduction in the number of the infected cells at 24 hours post inoculation, without changing the infected cell numbers during the initial phase of infection. These data indicate that the Le<sup>x</sup> structure could play a role in syncytial formation and cell-to-cell infection during the late phase of infection.

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 69(5), 405-413, 2016

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

Codes

  • NII Article ID (NAID)
    130005416321
  • NII NACSIS-CAT ID (NCID)
    AA1132885X
  • Text Lang
    ENG
  • ISSN
    1344-6304
  • NDL Article ID
    027626616
  • NDL Call No.
    Z53-C450
  • Data Source
    NDL  J-STAGE 
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