Interleukin-17A expression in human synovial mast cells in rheumatoid arthritis and osteoarthritis

• Kan Jun-ichiro

  Allergy and Immunology Project Team, Nihon University School of Medicine Department of Orthopaedic Surgery, Nihon University School of Medicine

• Mishima Shintaro

  Allergy and Immunology Project Team, Nihon University School of Medicine Department of Orthopaedic Surgery, Nihon University School of Medicine

• Kashiwakura Jun-ichi

  Allergy and Immunology Project Team, Nihon University School of Medicine Laboratory for Allergic Disease, RCAI, RIKEN Center for Integrative Medical Sciences (IMS-RCAI) Division of Medical Education Planning and Development, Nihon University School of Medicine

• Sasaki-Sakamoto Tomomi

  Allergy and Immunology Project Team, Nihon University School of Medicine Division of Medical Education Planning and Development, Nihon University School of Medicine

• Seki Masayuk

  Department of Orthopaedic Surgery, Nihon University School of Medicine

• Saito Shu

  Department of Orthopaedic Surgery, Nihon University School of Medicine

• Ra Chisei

  Department of Microbiology, Nihon University School of Medicine

• Tokuhashi Yasuaki

  Department of Orthopaedic Surgery, Nihon University School of Medicine

• Okayama Yoshimichi

  Allergy and Immunology Project Team, Nihon University School of Medicine Division of Medical Education Planning and Development, Nihon University School of Medicine

抄録

Background: Interleukin (IL)-17A plays a pivotal role in the pathogenesis of rheumatoid arthritis (RA). The expression of IL-17A in synovial mast cells (MCs) in RA and osteoarthritis (OA) has been reported, but the frequencies of IL-17A expression in synovial MCs have varied. The aim of this study was to investigate whether IL-17A expression is upregulated in human synovial MCs in RA and to elucidate the mechanism of IL-17A expression in synovial MCs. Methods: Synovial tissues were obtained from patients with RA or OA undergoing joint replacement surgery, and synovial MCs were enzymatically dispersed. Synovium-derived cultured MCs were generated by culturing synovial cells with stem cell factor. IL-17A expression was investigated using immunofluorescence in synovial tissues. IL-17A mRNA expression and its production from MCs were examined using RT-PCR and ELISA, respectively. Results: The number of IL-17A-positive ⁽⁺⁾ synovial MCs and the percentage of IL-17A⁺ MCs among all the IL-17A⁺ cells from RA patients were not significantly increased compared with those from OA subjects. The synovium-derived cultured MCs spontaneously released small amounts of IL-17A. Neither IgE- nor IgG-dependent stimulation increased IL-17A production from the MCs. IL-33, tumor necrosis factor-a, C5a, lipopolysaccharide or IL-23 plus IL-1β did not affect IL-17A production in MCs. Conclusions: The synovial MCs are not a main source of IL-17A in RA.

収録刊行物

• Allergology International

  Allergology International 65 (Supplement.1), 11-16, 2016

  一般社団法人日本アレルギー学会

キーワード

• Arthritis, Human mast cells, Interleukin-17, Osteoarthritis, Rheumatoid

詳細情報

• CRID: 1390282679609344512
• NII論文ID: 130005418947
• DOI: 10.1016/j.alit.2016.04.007
• ISSN: 14401592
• ISSN: 13238930
• Web Site: https://api.elsevier.com/content/article/PII:S1323893016300442?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S1323893016300442?httpAccept=text/plain
• 本文言語コード: en
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