<b>Induction of langerin</b><sup><b>+</b></sup><b> Langerhans cell-like cells expressing reduced TLR3 from CD34</b><sup><b>+</b></sup><b> cord blood cells stimulated with GM-CSF, TGF-β1, and </b><b>TNF-α </b>

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Abstract

<p>Langerhans cells (LCs), a subset of dendritic cells (DCs), reside in body surface presenting antigens from various pathogens and activate immune system after migrating to vicinal lymph nodes. We recently demonstrated that the E-cadherin interaction allowed peripheral blood (PB) CD14<sup>+</sup> cells to differentiate into LC-like cells that closely resemble primary LCs. Here, with a combination of GM-CSF, TGF-β, and TNF-α, we induced LC-like cells from umbilical cord blood (UCB)derived CD34<sup>+</sup> cells and compared them with those induced from PB CD14<sup>+</sup> cells. In contrast to PB CD14<sup>+</sup> cell-derived LC-like cells with an undetectable surface level of toll-like receptor (TLR)4 and an unresponsiveness feature to bacterial lipopolysaccharide (LPS), CB CD34<sup>+</sup> cellsderived LC like cells expressed a low, but apparent, surface level of TLR4 and a reduced level of intracellular TLR3. Consistent with this result, they responded to bacterial LPS, but poorly to poly(I:C) reflecting viral RNA. These findings suggest that LC-precursors from circulating PB CD14<sup>+</sup> cells seem to be arranged in the outer barrier of skin, while LC-precursors from local undifferentiated UCB-derived CD34<sup>+</sup> cells may be arranged in the inner barrier of mucosal tissues and work together to combat against external pathogens as well as internal malignancies throughout body surface.</p>

Journal

  • Biomedical Research

    Biomedical Research 37(5), 271-281, 2016

    Biomedical Research Press

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