A Simple Method for Ion Channel Recordings Using Fine Gold Electrode
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- OKUNO Daichi
- Laboratory for Cell Dynamics Observation, Quantitative Biology Center, Riken
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- HIRANO Minako
- The Graduate School for the Creation of New Photonics Industries
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- YOKOTA Hiroaki
- The Graduate School for the Creation of New Photonics Industries
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- ONISHI Yukiko
- Laboratory for Cell Dynamics Observation, Quantitative Biology Center, Riken
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- ICHINOSE Junya
- Graduate School of Natural Science and Technology, Okayama University
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- IDE Toru
- Graduate School of Natural Science and Technology, Okayama University
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Abstract
The artificial bilayer single-channel recording technique is commonly used to observe detailed pharmacological properties of various ion channel proteins. It permits easy control of the solution and membrane lipid composition, and is also compatible with pharmacological screening devices. However, its use is limited due to low measurement efficiency. Here, we develop a novel artificial bilayer single-channel recording technique in which bilayers are made and channels are reconstituted into the membranes by contacting a gold electrode to the lipid-solution interface. Using this technique, we measured the single-channel currents of two channel-forming peptides, gramicidin and alamethicin, and a channel-forming protein, α-hemolysin. This technique requires only one action, allowing the technique to potentially be combined with high-throughput screening devices.
Journal
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- Analytical Sciences
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Analytical Sciences 32 (12), 1353-1357, 2016
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204259902208
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- NII Article ID
- 130005433385
- 40021025518
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 027779076
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- PubMed
- 27941267
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed