Diversity of Methane-Oxidizing Bacteria in Soils from "Hot Lands of Medolla" (Italy) Featured by Anomalous High-Temperatures and Biogenic CO₂ Emission
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- Cappelletti Martina
- Department of Pharmacy and Biotechnology, University of Bologna
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- Ghezzi Daniele
- Department of Pharmacy and Biotechnology, University of Bologna
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- Zannoni Davide
- Department of Pharmacy and Biotechnology, University of Bologna
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- Capaccioni Bruno
- Department of Biological, Geological and Environmental Sciences, University of Bologna
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- Fedi Stefano
- Department of Pharmacy and Biotechnology, University of Bologna
書誌事項
- タイトル別名
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- Diversity of Methane-Oxidizing Bacteria in Soils from “Hot Lands of Medolla” (Italy) Featured by Anomalous High-Temperatures and Biogenic CO<sub>2</sub> Emission
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<p>“Terre Calde di Medolla” (TCM) (literally, “Hot Lands of Medolla”) refers to a farming area in Italy with anomalously high temperatures and diffuse emissions of biogenic CO2, which has been linked to CH4 oxidation processes from a depth of 0.7 m to the surface. We herein assessed the composition of the total bacterial community and diversity of methane-oxidizing bacteria (MOB) in soil samples collected at a depth at which the peak temperature was detected (0.6 m). Cultivation-independent methods were used, such as: i) a clone library analysis of the 16S rRNA gene and pmoA (coding for the α-subunit of the particulate methane monooxygenase) gene, and ii) Terminal Restriction Fragment Length Polymorphism (T-RFLP) fingerprinting. The 16S rRNA gene analysis assessed the predominance of Actinobacteria, Acidobacteria, Proteobacteria, and Bacillus in TCM samples collected at a depth of 0.6 m along with the presence of methanotrophs (Methylocaldum and Methylobacter) and methylotrophs (Methylobacillus). The phylogenetic analysis of pmoA sequences showed the presence of MOB affiliated with Methylomonas, Methylocystis, Methylococcus, and Methylocaldum in addition to as yet uncultivated and uncharacterized methanotrophs. Jaccard’s analysis of T-RFLP profiles at different ground depths revealed a similar MOB composition in soil samples at depths of 0.6 m and 0.7 m, while this similarity was weaker between these samples and those taken at a depth of 2.5 m, in which the genus Methylocaldum was absent. These results correlate the anomalously high temperatures of the farming area of “Terre Calde di Medolla” with the presence of microbial methane-oxidizing bacteria.</p>
収録刊行物
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- Microbes and environments
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Microbes and environments 31 (4), 369-377, 2016
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001204347222784
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- NII論文ID
- 130005434964
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- NII書誌ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 027806850
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- PubMed
- 27645100
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可