Electrochemical Control of Bioluminescence by Blocking the Adsorption of the Bacterial Luciferase Using a Mercaptobipyridine Self-assembled Monolayer

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  • YAMASAKI Shinya
    Faculty of Pure and Applied Sciences and Center for Research in Isotopes and Environmental Dynamics, University of Tsukuba
  • YAMADA Shuto
    Department of Applied Chemistry, School of Applied Sciences National Defense Academy
  • TAKEMURA Hiroyuki
    Department of Chemical and Biological Science, Faculty of Science, Japan Women’s University
  • TAKEHARA Kô
    Department of Chemistry, Kyushu University

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Abstract

An N-butyl-N′-(4-mercaptobutyl)-4,4′-bipyridinium (4BMBP) was modified on a gold electrode to improve the electrochemical control of the bacterial luciferase (BL) luminescence system. The 4BMBP-modified gold electrode (4BMBP/Au) was able to prevent the adsorption of BL on the electrode surface, and enhanced the electrochemical regeneration rate of the reduced flavin mononucleotide (FMNH2), which is one of the substrates of the BL luminescence reaction. By using the 4BMBP/Au, the luminescence intensity increased by about 27% compared to that of a bare gold electrode (bare Au). Moreover, the modified electrode improved the time required for analysis because the modified layer prevented BL adsorption. Even without a refreshing procedure for each measurement, a constant luminescence intensity could be observed, and the analysis time was reduced to half (about 10 min) for one sample. The 4BMBP/Au is not only useful to control of the BL luminescence system, but also for electrochemical measurements in the presence of proteins.

Journal

  • Analytical Sciences

    Analytical Sciences 33 (3), 307-311, 2017

    The Japan Society for Analytical Chemistry

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