Parathyroid cells express functional alpha 1C subunit of L-type calcium channel (CACNA1C)

• Matsuba Douchi

  Dept. Physiol., Jikei Univ. Sch. Med., Tokyo, Japan

• Suda Norio

  Dept. Physiol., Jikei Univ. Sch. Med., Tokyo, Japan

• Adachi-Akahane Satomi

  Lab. Cell Signaling, Univ. Tokyo Grad. Sch. Pharm. Sci.

• Yokoyama Keitaro

  Dept. Nephrol. & Hypertension, Jikei Univ. Sch. Med.

• Iida Rinako

  Dept. Nephrol. & Hypertension, Jikei Univ. Sch. Med.

• Ohkido Ichiro

  Dept. Nephrol. & Hypertension, Jikei Univ. Sch. Med.

• Hara Shino

  Dept. Nephrol. & Hypertension, Jikei Univ. Sch. Med.

• Takeyama Hiroshi

  Dept. Surgery, Jikei Univ. Sch. Med.

• Urashima Mitsuyoshi

  Dept. Clin. Res. Development, Jikei Univ. Sch. Med.

Bibliographic Information

Other Title

• 二次性機能亢進状態の副甲状腺細胞は心筋様L型カルシウムチャネルを発現する

Abstract

We asked whether CACNA1C is expressed in parathyroid cells in patients with secondary hyperparathyroidism (SHPTH). Immunohistochemical staining gave positive results for CACNA1C in the parathyroid cells. Parathyroid glands surgically removed from these patients were treated with the digestion buffer to isolate and culture the cells. PTH measurements confirmed secretion of PTH from the cultured cells. Cytoplasmic Ca²⁺ concentration of the fluo-3 AM loaded cells was measured with a modified Nipkow laser scanning confocal microscope system (CSU-21, Yokogawa Electric Company, Japan) attached to an inverted microscope (Olympus IX70). When the fluo-3 loaded cells were depolarized by exposure to 150 mM K⁺ solution, a transient increase in fluo-3 fluorescence was detected only in the presence of extracellular Ca²⁺. The fluo-3 Ca²⁺ transients were found to be sensitive to Ca²⁺ channel agonist, FPL64176 and a Ca²⁺ channel antagonist, nitrendipine. Depolarizing the cells under the whole-cell configuration also evoked inward Ca²⁺ currents, which showed current-voltage relationship similar to that of L-type Ca²⁺ channels (peak current around 0 mV). These results suggest that CACNA1C is expressed in parathyorid cells in patients with SHPTH. [Jpn J Physiol 55 Suppl:S207 (2005)]

Journal

• Proceedings of Annual Meeting of the Physiological Society of Japan

  Proceedings of Annual Meeting of the Physiological Society of Japan 2005 (0), S207-S207, 2005

  PHYSIOLOGICAL SOCIETY OF JAPAN

Keywords

• parathyroid
• calcium channel
• calcium
• PTH
• dihydropyridine receptor

Details

• CRID: 1390282680705611648
• NII Article ID: 130005447810
• DOI: 10.14849/psjproc.2005.0_s207_3
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed