Serum-dependence of AMPA receptor-mediated proliferation in glioma cells
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- Yoshida Yukari
- Dept. Neurophysiol., Gunma Univ. Grad. Sch. Med., Maebashi, Gunma, Japan
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- Tsuzuki Keisuke
- Dept. Neurophysiol., Gunma Univ. Grad. Sch. Med., Maebashi, Gunma, Japan
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- Ishiuchi Shogo
- Dept. Neurosurg., Gunma Univ. Grad. Sch. Med., Maebashi, Gunma, Japan
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- Ozawa Seiji
- Dept. Neurophysiol., Gunma Univ. Grad. Sch. Med., Maebashi, Gunma, Japan
Bibliographic Information
- Other Title
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- グリオーマ細胞におけるAMPA受容体を介した細胞増殖の血清依存性
Abstract
Glutamate may cause Ca2+ entry through Ca2+-permeable glutamate receptors, which in turn stimulates the anti-apoptotic signaling cascade in glioma cells. Here, we found that a human glioma cell line, U-87 MG, expressed GluR1, GluR2 and GluR3 subunits of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate acid-type glutamate receptors (AMPARs). Approximately 20% of GluR2 was expressed in the unedited form, which is known to assemble Ca2+-permeable AMPARs. Ca2+ entry through the activation of these receptors by AMPA was detected clearly in approximately 20% of U-87 MG glioma cells. Cell proliferation assays revealed that the application of AMPA or glutamate facilitated cell proliferation by activating AMPARs in low-serum medium containing 0.5% fetal calf serum (FCS). Unexpectedly, cell proliferation by the activation of AMPARs was not detected in serum-rich medium containing 10% FCS. Overexpression of the unedited form of GluR2 (GluR2Q) by adenoviral-mediated gene transfer markedly increased the Ca2+ entry into U-87 MG cells. This treatment in the presence of glutamate facilitated proliferation and migration of U-87 MG cells in the low-serum condition, whereas it had again no effect in the serum-rich condition. It is therefore likely that cell proliferation and migration of U-87 MG cells are under the regulation of growth factors contained in the serum as well as Ca2+ entry through AMPARs, and that the latter regulation becomes evident only when serum factors are deprived of culture medium. [J Physiol Sci. 2006;56 Suppl:S117]
Journal
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- Proceedings of Annual Meeting of the Physiological Society of Japan
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Proceedings of Annual Meeting of the Physiological Society of Japan 2006 (0), 117-117, 2006
PHYSIOLOGICAL SOCIETY OF JAPAN
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Keywords
Details 詳細情報について
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- CRID
- 1390282680705186176
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- NII Article ID
- 130005448278
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed