Methyl p-hydroxybenzoate (methyl paraben) causes pain sensation through TRPA1 activation

Fujita Fumitaka, Moriyama Tomoko, Higashi Tomohiro, Shima Asako, Tominaga Makoto

doi:10.14849/psjproc.2007.0_099_3

Bibliographic Information

Other Title

メチルパラベンはTRPA1の活性化を介して痛みを引き起こす

Abstract

Parabens are commonly added in pharmaceutical, cosmetic and food products because of their wide antibacterial properties, low toxicity, inertness and chemical stability although the molecular mechanism of their antibacterial effect is not fully understood. Allyl isothiocyanate, cinnamaldehyde and allicin have strong antibacterial activities and are known to activate TRPA1 ion channel, which belongs to the TRP super family and is involved in nociception. Here we show that methyl p-hydroxybenzoate (methyl paraben) activates TRPA1 in both HEK293 cells expressing TRPA1 and in mouse sensory neurons with an EC₅₀ value of 4.4 mM, an easily attainable concentration in methyl paraben-containing products. Furthermore, methyl paraben caused pain-related behaviors in mice similar to those caused by allyl isothiocyanate, which was blocked by the TRP channel blocker, ruthenium red. These results indicate methyl paraben is able to activate TRPA1 and can cause pain sensation. As such, methyl paraben provides a useful tool for investigating TRPA1 function and development of antinociceptive agents acting on TRPA1. [J Physiol Sci. 2007;57 Suppl:S99]

Journal

Proceedings of Annual Meeting of the Physiological Society of Japan

Proceedings of Annual Meeting of the Physiological Society of Japan 2007 (0), 099-099, 2007

PHYSIOLOGICAL SOCIETY OF JAPAN

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Details 詳細情報について

CRID: 1390001205729015808

NII Article ID: 130005448854

DOI: 10.14849/psjproc.2007.0_099_3

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