The expression of short-chain fatty acid receptor, GPR41 in the human colon

• Tazoe Hideaki

  Inst. Env. Sci., Grad. Sch. Nut. Env. Sci., Univ. Shizuoka, Shizuoka, Japan

• Otomo Yasuko

  Inst. Env. Sci., Grad. Sch. Nut. Env. Sci., Univ. Shizuoka, Shizuoka, Japan

• Kaji Izumi

  Inst. Env. Sci., Grad. Sch. Nut. Env. Sci., Univ. Shizuoka, Shizuoka, Japan

• Ono Shigeyuki

  Kao Corporation, Tokyo, Japan

• Karaki Shin-ichiro

  Inst. Env. Sci., Grad. Sch. Nut. Env. Sci., Univ. Shizuoka, Shizuoka, Japan

• Kuwahara Atsukazu

  Inst. Env. Sci., Grad. Sch. Nut. Env. Sci., Univ. Shizuoka, Shizuoka, Japan

Bibliographic Information

Other Title

• ヒト大腸における短鎖脂肪酸受容体ＧＰＲ４１の発現について

Abstract

Short-chain fatty acids (SCFAs) are the major anions in the lumen of the large intestine. SCFAs are produced by bacterial fermentation of undigested carbohydrates and proteins. Luminal SCFAs are not only absorbed as nutrients, but also influence various physiological and pathophysiological functions of the gastrointestinal tract. Such effects of SCFAs in the intestinal lumen are considered to be induced via the activation of specific receptors and/or via absorption in epithelial cells. However, the sensing mechanism of SCFAs in the intestinal lumen is currently unclear. Nowadays, SCFA receptors were identified from orphan G-protein coupled receptors, specifically GPR41 and GPR43. We have reported the expression of GPR43 in the human colon. In this study, we investigated the expression of GPR41 in the human colon used by RT-PCR analysis, Western blot analysis and immunohistochemistry. As a result, we have discovered GPR41 mRNA and protein were expressed in mucosa of human colon, and the co-localization study demonstrated that GPR41-immunoreactive enteroendocrine cells contained peptide YY, but not serotonin. These results are similar to another SCFA receptor, GPR43. Therefore, it is considered that SCFAs are recognized by GPR41- and/or GPR43-immunoreactive enteroendocrine cells, which secrete peptide YY. Peptide YY is also known to be an important appetite control hormone, inhibiting food intake as a satiety signal. There is a possibility that luminal SCFAs may influence appetite control. [J Physiol Sci. 2008;58 Suppl:S85]

Journal

• Proceedings of Annual Meeting of the Physiological Society of Japan

  Proceedings of Annual Meeting of the Physiological Society of Japan 2008 (0), 085-085, 2008

  PHYSIOLOGICAL SOCIETY OF JAPAN

Keywords

• short-chain fatty acid
• GPR41
• PYY

Details

• CRID: 1390001205730130688
• NII Article ID: 130005449544
• DOI: 10.14849/psjproc.2008.0_085_3
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed