Sexual difference of eggshell hormone contents during the early post hatch period in the Japanese quail (Coturnix coturnix japonica)

• QASIMI Mohammad Ibrahim

  Basic Veterinary Science, United Graduate of Veterinary Science, Gifu University Veterinary Physiology, Tokyo University of Agriculture and Technology

• MOHIBBI Hadi

  Basic Veterinary Science, United Graduate of Veterinary Science, Gifu University Veterinary Physiology, Tokyo University of Agriculture and Technology

• NAGAOKA Kentaro

  Basic Veterinary Science, United Graduate of Veterinary Science, Gifu University Veterinary Physiology, Tokyo University of Agriculture and Technology

• WATANABE Gen

  Basic Veterinary Science, United Graduate of Veterinary Science, Gifu University Veterinary Physiology, Tokyo University of Agriculture and Technology

抄録

Identification of sex has a crucial role for poultry industries, birder and ecologist. In this study, we measured the hormonal content in eggshells during the post hatching to check whether it make possible to identify sex or not. If there is a difference in the amounts of sex hormones, it will be an alternative way to estimate sex ratio in a habitat especially for precocial birds. [Materials and methods] Eggshells were collected, dried, pulverized and mixed with 80% methanol. After centrifugation at 3000 rpm for 10 min, the supernatant samples were refrigerated at –20ºC for hormonal measurements by RIAs. [Results] In the present study, testosterone (T), estradiol (E2) and corticosterone (CORT) were measured. T and CORT concentration were detected at all stages in the eggshell and had contrary relationship to each other. T level was significantly high (p<0.01) in fresh eggshell and low at the 15^th d incubation and post hatched. Unlike to T result, CORT level was low after laying and gradually increased till hatching. During hatching CORT level was significantly different (p<0.01) between male and female eggshell. E2 was undetected since after laying, unless it was high at the 15^th d incubation and significantly high in female embryo (p<0.05). [Conclusion] Hatching time is kind of stressful condition for chicks. At this time chicks need to overcome this situation by increasing the stress hormone to expense more energy to survive. High CORT level may suppress the reproductive hormones. Further study is needed to verify the CORT level in male and female chicks’ eggshell and its effect on progesterone level.

収録刊行物

• 日本繁殖生物学会　講演要旨集

  日本繁殖生物学会　講演要旨集 107 (0), OR2-18-OR2-18, 2014

  公益社団法人 日本繁殖生物学会

詳細情報

• CRID: 1390001205715044736
• NII論文ID: 130005475180
• DOI: 10.14882/jrds.107.0_or2-18
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可