Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution
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- Sugano Mikio
- Department of Cardiovascular Surgery, Graduate School of Biomedical Sciences, Tokushima University
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- Yoshida Homare
- Department of Cardiovascular Surgery, Graduate School of Biomedical Sciences, Tokushima University
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- Kurobe Hirotsugu
- Department of Cardiovascular Surgery, Graduate School of Biomedical Sciences, Tokushima University
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- Arase Hiroki
- Department of Cardiovascular Surgery, Graduate School of Biomedical Sciences, Tokushima University
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- Kinoshita Hajime
- Department of Cardiovascular Surgery, Graduate School of Biomedical Sciences, Tokushima University
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- Kitaichi Takashi
- Department of Cardiovascular Surgery, Graduate School of Biomedical Sciences, Tokushima University
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- Sugasawa Noriko
- Department of Cardiovascular Surgery, Graduate School of Biomedical Sciences, Tokushima University
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- Nakayama Soichiro
- Department of Gynecology, Graduate School of Biomedical Sciences, Tokushima University
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- Maeda Kazuhisa
- Department of Gynecology, Graduate School of Biomedical Sciences, Tokushima University
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- Irahara Minoru
- Department of Gynecology, Graduate School of Biomedical Sciences, Tokushima University
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- Kitagawa Tetsuya
- Department of Cardiovascular Surgery, Graduate School of Biomedical Sciences, Tokushima University
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抄録
<p>Objectives: To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. Methods: MCT was administered to BALB/c Slc-nu/nu mice, and PH was induced in mice 4 weeks later. Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice. The medial thickness of pulmonary arterioles, ratio of right ventricular to septum plus left ventricular weight (RV/S+LV), and ratio of acceleration time to ejection time of pulmonary blood flow waveform (AT/ET) were determined 4 weeks after hUCB-MNC transplantation. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung by fluorescent microscopy. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioluminescence images. Results: Medial thickness, RV/S+LV and AT/ET were significantly improved 4 weeks after hUCB-MNC transplantation compared with those in mice without hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation. Conclusions: The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH. J. Med. Invest. 64: 43-49, February, 2017</p>
収録刊行物
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- The Journal of Medical Investigation
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The Journal of Medical Investigation 64 (1.2), 43-49, 2017
国立大学法人 徳島大学医学部