Novel Artificial Coenzyme Based on the Viologen Derivative for CO₂ Reduction Biocatalyst Formate Dehydrogenase

• Ikeyama Shusaku

  Graduate School of Science, Osaka City University

• Amao Yutaka

  Graduate School of Science, Osaka City University The Advanced Research Institute for Natural Science and Technology, Osaka City University Research Center for Artificial Photosynthesis (ReCAP), Osaka City University

Abstract

Formate dehydrogenase (FDH) is an attractive biocatalyst for CO₂ reduction. The reduced form of methylviologen acts as an artificial coenzyme for FDH. For further improvement in the CO₂ reduction catalytic activity of FDH, a viologen derivative with two amino groups, 1,1′-diaminoethyl-4,4′-bipyridinium salt (DA²⁺), was synthesized as the effective artificial coenzyme for FDH. By enzyme kinetic analysis, the catalytic efficiency, i.e., k_cat/K_m value of the reduced form of DA²⁺ was estimated to be more than 560 times larger than that of NADH, the natural coenzyme for FDH.

Journal

• Chemistry Letters

  Chemistry Letters 45 (11), 1259-1261, 2016

  The Chemical Society of Japan

Keywords

• CO₂ reduction
• Viologen derivative
• Artificial coenzyme

Details

• CRID: 1390282679564107392
• NII Article ID: 130005509446
• DOI: 10.1246/cl.160687
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://www.journal.csj.jp/doi/pdf/10.1246/cl.160687
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed