A Versatile and Rapidly Deployable Device to Enable Spatiotemporal Observations of the Sessile Microbes and Environmental Surfaces

• Kiyokawa Tatsunori

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Usuba Ryo

  Graduate School of Pure and Applied Sciences, University of Tsukuba

• Obana Nozomu

  Faculty of Life and Environmental Sciences, University of Tsukuba

• Yokokawa Masatoshi

  Faculty of Pure and Applied Sciences, University of Tsukuba

• Toyofuku Masanori

  Faculty of Life and Environmental Sciences, University of Tsukuba Department of Plant and Microbial Biology, University of Zurich

• Suzuki Hiroaki

  Faculty of Pure and Applied Sciences, University of Tsukuba

• Nomura Nobuhiko

  Faculty of Life and Environmental Sciences, University of Tsukuba

Abstract

Although microbes typically associate with surfaces, detailed observations of surface-associated microbes on natural substrata are technically challenging. We herein introduce a flow channel device named the Stickable Flow Device, which is easily configurable and deployable on various surfaces for the microscopic imaging of environmental microbes. We demonstrated the utility of this device by creating a flow channel on different types of surfaces including live leaves. This device enables the real-time imaging of bacterial biofilms and their substrata. The Stickable Flow Device expands the limits of conventional real-time imaging systems, thereby contributing to a deeper understanding of microbe-surface interactions on various surfaces.

Journal

• Microbes and Environments

  Microbes and Environments 32 (1), 88-91, 2017

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• flow channel device
• real-time imaging
• three-dimensional imaging
• biofilms
• microbe-surface interactions

Details

• CRID: 1390282679323081856
• NII Article ID: 130005519591
• NII Book ID: AA11173196
• DOI: 10.1264/jsme2.me16161
• ISSN: 13474405
• ISSN: 13426311
• HANDLE: 2241/00146067
• NDL BIB ID: 028095498
• PubMed: 28321008
• Web Site: https://tsukuba.repo.nii.ac.jp/records/41118
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028095498
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/32/1/32_ME16161/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed